Hana El‐Samad

The paper introduces macroscopic metrics to analyze RNA-seq data and reveal chromatin modifications in aging and disease, showing their sensitivity to chromatin structure changes and effectiveness in distinguishing cell states.

This paper introduces bioPROTACs, a genetic tool to control CAR T cell signaling by degrading specific proteins, demonstrating its effectiveness in inhibiting CAR signaling in human T cells.

The paper characterizes small molecule and cell contact-inducible systems for controlling gene expression and differentiation in mouse embryonic stem cells.

This study demonstrates that de novo-designed minibinders can be adapted to various cell engineering tools, improving antigen detection and cytotoxicity compared to antibody-based systems.

The paper introduces CoRa, a framework to quantify biological feedback control by comparing systems with and without feedback.

The paper uses optogenetic control of RelA to show its nuclear translocation alone can activate downstream genes, revealing dynamic gene responses and the need for complex promoter models.

This paper introduces SARSNotch, a sentinel cell system that detects SARS-CoV-2 Spike protein and triggers genetic responses in cell lines and T lymphocytes.

The paper introduces TuNR, a synthetic circuit that independently controls mean and variability of gene expression in human cells, enabling study of cell-to-cell heterogeneity.

The paper identifies genes with biphasic expression in response to increasing cAMP levels and shows PKA and ERK1/2 exhibit opposite biphasic responses, revealing complex signaling integration.

The paper introduces CLASP, an optogenetic tool to control TF localization, and shows differential promoter responses to pulsatile vs. continuous TF inputs.

The paper shows that accurate prediction of genetic circuit behavior requires multidimensional characterization of parts, using computational models and experimental verification.

The paper shows that removing stress-induced cell cycle arrest in yeast accelerates osmotic shock adaptation but increases vulnerability to subsequent stress.

The paper introduces MTK, a toolkit for rapid modular DNA circuit assembly in mammalian cells, demonstrating its use in constructing an Ebola virus replication complex circuit.

This paper designs and analyzes a biochemical PID controller using biological molecules, showing improved performance through computational and analytical methods.

Inserting theophylline aptamers into the sgRNA yields ligand-activated and ligand-deactivated guides that tune CRISPRi in E. coli over 11x to 13x dynamic ranges, enabling two-ligand multiplexing.

The paper presents a dataset analyzing kinase activity and gene expression in yeast under environmental stress, revealing how kinases modulate stress responses.

This study introduces CLOC, a method that uses real-time optogenetic compensation to define the dynamic requirements of feedback regulators in yeast.

This paper introduces complexity-aware simple modeling to bridge the gap between small-scale and large-scale biological models by extracting robust principles despite unknown interactions.