Karen N. Allen

Glycoconjugate diversification in Campylobacter concisus is determined by two glycosyltransferases

The paper identifies the final N-linked glycan structure in Campylobacter concisus and reveals the roles of two glycosyltransferases in its diversification.

Deciphering AMP deaminase-2 structure, activators and regulators underpinning cellular function in human fructose and nucleotide metabolism

This study characterizes the structure and regulation of hAMPD2-2, revealing ATP activation, GTP inhibition, and Pi's role in modulating the enzyme under physiological and fructose conditions.

Detergent exchange from lipid nanoparticles into detergent micelles unlocks a tool for biochemical and kinetic characterization of membrane proteins

This paper introduces a detergent exchange method to transfer membrane proteins from lipid nanoparticles to detergent micelles, preserving function and homogeneity for biochemical analysis.

A nicotine biosensor derived from microbial screening

The paper develops a nicotine biosensor using a microbial-derived redox enzyme, demonstrating detection over a clinically relevant range.

Wearable sweat nicotine biosensor based on a nicotineoxidoreductase and its natural electron acceptor

This paper develops a wearable nicotine biosensor using NicA2 and CycN, achieving high sensitivity and selectivity for real-time sweat monitoring.

Selection of nanobodies against liponanoparticle-embedded membrane proteins by yeast surface display

This paper develops a method to select nanobodies against membrane proteins embedded in liponanoparticles using yeast surface display, achieving high-affinity and stable Nb-protein complexes.

Structural Basis for the Improved RNA-Clamping of Amidino-Rocaglates to eIF4A1

This paper presents the X-ray structure of an amidino-rocaglate in complex with eIF4A1 and RNA, showing how its rigid scaffold improves RNA-clamping compared to rocaglamide A.

Proteome-Wide Bioinformatic Annotation and Functional Validation of the Monotopic Phosphoglycosyl Transferase Superfamily

The paper identifies and validates substrate specificity of bacterial monotopic phosphoglycosyl transferases using bioinformatics and in vitro assays, supporting inhibitor discovery for antibiotic development.

A Conserved Core Region of the Scaffold NEMO is Essential for Signal-induced Conformational Change and Liquid-liquid Phase Separation

This study identifies a conserved core region in NEMO essential for conformational changes and liquid-liquid phase separation in the NF-κB signaling pathway.