Dominic Johnson

Localised negative feedback shapes genome-wide patterning of meiotic DNA breaks

The paper models how Tel1-mediated feedback shapes Spo11-DSB formation in yeast, showing local inhibition leads to genome-wide DSB redistribution.

Meiotic prophase length modulates Tel1-dependent DNA double-strand break interference

The paper shows that extending meiotic prophase reduces DSB clustering by allowing chromosomal domains to reach similar Spo11-DSB potential.

Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri

The paper shows that absence of Red1 and Hop1 recruitment prevents meiotic recombination on a chromosome arm containing the sex locus in Lachancea kluyveri, affecting genome evolution and fitness.

Concerted cutting by Spo11 illuminates DNA break mechanisms and initiates gap repair during meiosis

The paper shows that DNA double-strand breaks (DSBs) during meiosis often occur in clusters with periodic spacing, challenging the idea that DSBs are isolated events.

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

The paper maps Top2-linked DNA breaks with nucleotide resolution in yeast and human genomes, revealing distinct activity fractions and sequence influences on cleavage.

Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2

This study shows TDP2 can remove Spo11 from Spo11-DSBs in vitro but not in vivo, suggesting structural barriers prevent TDP2 access to Spo11-DNA in living cells.