Michael P. Washburn

The paper uses an integrative approach to model the structure of an IDR-driven HDAC2 complex, revealing interactions not captured by AlphaFold alone.

The paper builds structural models of HDAC1/2-containing chromatin remodeling complexes using XL-MS and integrative modeling, revealing how the CTD IDR of HDAC1 folds into alpha helices.

This study integrates transcriptomic, proteomic, and phosphoproteomic data from two pediatric B-ALL subtypes to identify subtype-specific regulatory interactions and potential therapeutic targets.

The paper investigates how dual-role regulators interact with the Sin3 complex using structural and functional analyses, revealing mechanisms for transcriptional crosstalk.

The paper identifies TFIID and SAGA complex subunits in C. albicans and shows that TAF12 paralogs form heterodimers through cotranslational assembly, ensuring specificity and stability of histone-like complexes.

This study evaluates the use of FAIMS with Orbitrap Ascend Tribrid Mass Spectrometer to enhance AP-MS analysis of SAP25 protein complexes, showing improved sensitivity and reduced contaminants.

This study identifies DCLK1's role in regulating invadopodia dynamics and MMP trafficking in HNSCC, showing its impact on invasion and metastasis.

The paper identifies how the BRCA1-Y1853ter mutation alters protein interactions and cellular processes, suggesting gain-of-function effects in cancer.

The paper identifies distinct regions of SAP25 that recruit O-GlcNAc transferase, TET methylcytosine dioxygenases, and ubiquitin ligase/hydrolase activities to the Sin3/HDAC complex.

This study identifies DYRK1A's role in promoting mTORC1 activity by inhibiting TSC complex activity through phosphorylation of TSC2, and shows conservation in Drosophila.

This study shows that loss of GPR52 in breast cancer cells increases cell clustering, EMT features, and tumor burden in zebrafish, suggesting GPR52 as a potential therapeutic target.

This study identifies FAM60A as a regulator of RNA splicing, showing it interacts with HDAC1 and spliceosome components, expanding its known role beyond the Sin3/HDAC complex.

The paper introduces SCAP, a method combining affinity purification, live cell imaging, and cross-linking mass spectrometry to build integrative structural models of protein complexes.

The paper applies the TopS method to flow cytometry data to identify rare cell populations in a human hematopoietic stem cell dataset.

The paper investigates how paralog switching affects Sin3 complex interaction networks in humans, revealing shared and distinct interactions between SIN3A and SIN3B.

The paper maps the WDR76 interactome using AP-MS and identifies key interactions with the CCT complex and other proteins, validating interactions with GAN and SIRT1.

The paper introduces TopS, a method to score protein interactions in proteomic datasets, revealing direct interactions and modules in human and yeast networks.

This paper identifies DYRK1A's role in DNA repair by regulating RNF169-mediated 53BP1 recruitment to DSB sites, with implications for DNA damage therapy response.

The paper identifies a novel protein complex, WHHERE, that regulates retinoic acid signaling and embryonic symmetry by controlling histone modifications and RNA Polymerase II recruitment.