Olivier Pourquié

This study compares 3D skeletal muscle spheroids (iSMS) with 2D cultures for modeling Duchenne Muscular Dystrophy, finding improved PAX7 expression and altered Notch signaling in iSMS.

Human Developmental Cell Atlas integrates ~4.6 million cells/nuclei from 4-22 post-conception weeks into 454 refined cell types and maps 114 spatial niches across 6PCW whole-embryo sections.

This study generates human ventral skin and amnion-like organoids by adjusting signaling and cellular composition, revealing key developmental pathways and interactions.

This study examines how different exercise intensities affect the human plasma secretome and interorgan communication through multi-cohort interventions and multi-organ gene expression analysis.

This study identifies a proteomic signature in humans following acute cold exposure that correlates with cardioprotective and anti-aging effects.

The paper shows that active tissue expansion, driven by FGF signaling and HA production, propels vertebrate body elongation without relying on cell proliferation.

The paper identifies a brain-wide twisting asymmetry in mice, similar to human cerebral petalia, using data from 3,500 animals across six cohorts.

This study identifies hemispheric asymmetry in mouse brains, revealing species-specific patterns and differences from humans, with implications for autism research.

The paper identifies mechanical cues influencing epiblast cell fate in chicken embryos through single-cell RNA sequencing and lineage tracing.

This paper introduces a MALDI MSI method to spatially profile metals in tissues, applying it to study embryogenesis, genetic disorders, and chemotherapy delivery.

This study reprograms non-limb fibroblasts into cells with limb progenitor properties using Prdm16, Zbtb16, Lin28, and Lin41.

This study uses single-cell RNA sequencing to identify brown fat precursors and demonstrates their in vitro differentiation into functional brown adipocytes.

This paper introduces TiFM, a technique for measuring and applying forces to small tissues in early chicken embryos, showing it can capture stress dynamics and alter morphogenetic processes.

Zebrafish embryos sequenced individually across ten developmental stages, paired with light-sheet lineage reconstruction, form an atlas and browser for in silico fate mapping.

The paper shows that metabolic rates influence developmental speed in mouse and human embryos, with mouse cells having higher metabolic rates and faster segmentation clock periods.

This study introduces 3D culture systems to model human somitogenesis, revealing the role of the segmentation clock in establishing somite compartments.

This study shows that mechanical tension drives multi-scale self-organisation in human iPSC-derived muscle fibers by coordinating sarcomere assembly and myofiber bundling.

This study uses bulk and single-cell assays to analyze gene expression, histone modifications, and chromatin features in ESCs transitioning to mesoderm lineages, identifying cis-regulatory elements and transcription factors involved in lineage specification.

This study generates human paraxial mesoderm organoids that form somite-like structures in vitro, enabling analysis of human spinal cord development.

This study shows that cells in the anterior primitive streak of chicken embryos contribute to both neural and mesodermal lineages, with bipotential fate delayed and maintained by specific proliferation and ingression patterns.

The paper shows that mechanical interactions coordinate the elongation of axial and paraxial tissues in avian embryos through a feedback loop involving cell motility and compression.

The paper identifies a human segmentation clock by showing human PSM cells exhibit Notch and YAP-dependent oscillations of HES7 with a 5-hour period.

The paper shows that Retinoic Acid signaling, specifically through Rere, controls brain asymmetry and handedness in zebrafish and mouse.

This study shows Hox genes control avian forelimb position through timed collinear activation during gastrulation and subsequent regulation of limb initiation.

The paper shows that aerobic glycolysis increases intracellular pH in tail bud cells, promoting β-catenin acetylation and Wnt signaling, which drives mesodermal fate over neural fate.

This study develops an in vitro protocol to recapitulate early paraxial mesoderm development in mouse ES cells, showing efficient differentiation into muscle precursor cells and functional muscle fibers.

The paper identifies a novel protein complex, WHHERE, that regulates retinoic acid signaling and embryonic symmetry by controlling histone modifications and RNA Polymerase II recruitment.

The paper quantifies cell motility gradients in the chicken embryo's Tail Bud and shows that mechano-chemical models explain body elongation.

This study shows that TAF10 is not essential for early somitogenesis but is critical for lateral plate differentiation in the mouse embryo.

The paper shows that Warburg-like metabolism in the posterior region of embryos coordinates FGF and Wnt signaling to promote embryonic axis elongation.