Peter M. Pryciak

Molecular basis of Cyclin B1-CDK1 recruitment by MAD1

The paper identifies a non-canonical helical binding motif in MAD1 that specifically interacts with Cyclin B1 to recruit CDK1 to the SAC, revealing a mechanism for B-type cyclin recognition.

An Atlas of Short Linear Motif-Mediated Human Protein-Protein Interactions

The paper maps over 20,000 human protein-protein interactions mediated by short linear motifs using a peptide library screening approach.

High-throughput discovery and deep characterization of cyclin-CDK docking motifs

The paper identifies and characterizes cyclin-CDK docking motifs through high-throughput peptide binding assays and structural analysis, revealing a broader repertoire of motif types and binding mechanisms.

A quantitative intracellular peptide binding assay reveals recognition determinants and context dependence of short linear motifs

The paper introduces SIMBA, a functional assay to quantify SLiM binding, showing in vivo binding strength correlates with in vitro affinity and revealing context-dependent recognition determinants.

A novel multifunctional role for Hsp70 in binding post-translational modifications on clients

This paper uses cross-linking mass spectrometry to characterize Hsp70 interactions in budding yeast, revealing self-association, multi-point client interactions, and novel PTMs critical for client function.

Comprehensive analysis of fungal G1 cyclin docking motif sequences that control CDK regulatory potency in vivo

The paper analyzes fungal G1 cyclin docking motifs to determine their sequence features and regulatory potency in vivo.