This paper develops fluorescently labeled GagPol variants for live-cell imaging of HIV-1 assembly, enabling single-cell analysis of Gag and GagPol interactions and dynamics.

bioRxiv · Jul 22, 2026 · PreprintBiophysics

Resolving the Dynamic Interplay of the Conformational States in the TPR Domain of Human AMPylase FicD

Svenja Runge, Ecenaz Bilgen, Andrea Magni, Giorgio Bonollo, Giorgio Colombo, Aymelt Itzen, Don C. Lamb

This study uses smFRET to analyze conformational dynamics of FicD's TPR motifs, showing equilibrium between TPR-in and TPR-out states influenced by ATP and BiP.

Proceedings of the National Academy of Sciences · Aug 21, 2025Peer-reviewedBiophysics

Triple labeling resolves a GPCR intermediate state by 3-color single molecule FRET

Léo Bonhomme, Ecenaz Bilgen, Caroline Clerté, Jean‐Philippe Pin, Philippe Rondard, Emmanuel Margeat, Don C. Lamb, Robert B. Quast

This paper introduces a triple-labeling strategy for single molecule FRET to study conformational changes in GPCRs, revealing a previously unknown intermediate state.

Journal of the American Chemical Society · May 15, 2025Peer-reviewedBiophysics

Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive demethylation in mammals

Christopher B. Mulholland, Atsuya Nishiyama, Joël Ryan, Ryohei Nakamura, Merve Yiğit, Ivo M. Glück, Carina Trummer, Weihua Qin, Michael D. Bartoschek, Franziska R. Traube, Edris Parsa, Enes Ugur, Miha Modic, Aishwarya Acharya, Paul Stolz, , Heinrich Leonhardt

The paper identifies DPPA3 as a driver of genome-wide passive DNA demethylation in mammals, showing it can bypass TET protein requirements and function across species.

bioRxiv · Apr 4, 2020 · PreprintDevelopmental Biology