Heinrich Leonhardt

Heterochromatin organization and liquid-liquid phase separation: it is not about if but about when

The paper investigates how heterochromatin proteins form barrier compartments in cells, showing differences between proteins, cell types, and compartments, and proposes a model based on protein accumulation and competition.

Mechanisms of gene regulation by SRCAP and H2A.Z

The paper investigates the distinct roles of SRCAP and H2A.Z in gene regulation during the cell cycle in pluripotent stem cells, revealing SRCAP's H2A.Z-independent inhibitory function and H2A.Z's repressive role in lineage-specific gene expression.

Nanoscale Dynamics of Enhancer-Promoter Interactions during Exit from Pluripotency

The paper uses super-resolution microscopy to measure enhancer-promoter distances during pluripotency exit, finding minimal changes despite large transcriptional changes.

Super-resolution compatible DNA labeling technique reveals chromatin mobility and organization changes during differentiation

The paper develops a DNA labeling technique to study chromatin mobility and organization during neural differentiation, finding reduced chromatin mobility and increased compaction in differentiated cells.

TUB-010, a novel anti-CD30 antibody-drug conjugate based on Tub-tag technology, widens the therapeutic window by reducing toxicity while maintaining high efficacy

TUB-010 is an anti-CD30 ADC using Tub-tag conjugation technology that reduces toxicity and improves therapeutic window compared to Adcetris.

TET dioxygenases localize at splicing speckles and promote RNA splicing

This study shows TET dioxygenases localize at splicing speckles and promote RNA splicing through interactions with splicing factors and cytosine modifications.

Microscopy analysis of delayed co-transcriptional splicing in highly expressed thyroglobulin gene

The paper uses microscopy to study delayed co-transcriptional splicing in the highly expressed thyroglobulin gene, finding a delay in splicing downstream of transcribed introns.

Nucleoporin Nup153 docks the splicing machinery to the nuclear pore for efficient mRNA processing

This study identifies Nup153 as a docking point for splicing machinery at the nuclear pore, showing its role in mRNA processing and gene splicing.

Generation of densely labeled oligonucleotides for the detection of small genomic elements

This paper introduces a method to generate densely labeled oligonucleotides for detecting small genomic elements using FISH in single cells.

Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

This study shows UHRF1 has non-canonical roles beyond DNMT1 activation in maintaining DNA methylation homeostasis in cancer cells.

Capture of Post-Pluripotency Human Neuromesodermal and Neural Tube Axial Stem Cell States

The paper captures two human axial stem cell states and identifies regulatory networks and mechanisms underlying their maintenance and differentiation.

Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor

This study uses a small molecule inhibitor to rapidly and synchronously induce senescence-like features in human cells, enabling analysis of its core regulatory program without paracrine effects.

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes

SPARCS enables genome-wide CRISPR screening for spatial cellular phenotypes by combining laser microdissection with deep learning, identifying key genes involved in autophagosome formation.

The termination of UHRF1-dependent PAF15 ubiquitin signaling is regulated by USP7 and ATAD5

This study identifies USP7 and ATAD5 as key factors in terminating UHRF1-dependent PAF15 ubiquitin signaling, linking this process to the completion of maintenance DNA methylation.

Epiblast morphogenesis is controlled by selective mRNA decay triggered by LIN28A relocation

The paper identifies LIN28A as a key factor in mRNA decay during epiblast morphogenesis, linking cell identity changes to embryonic growth through nucleo-cytoplasmic translocation.

FUS-dependent liquid-liquid phase separation is an early event in double-strand break repair

This study shows that FUS-dependent liquid-liquid phase separation is essential for early DNA damage response activation and repair complex assembly.

An Xist-dependent protein assembly mediates Xist localization and gene silencing

The paper shows that Xist RNA forms a protein condensate with binding proteins to establish the Xi-compartment and maintain gene silencing.

Deciphering the regulatory logic of a Drosophila enhancer through systematic sequence mutagenesis and quantitative image analysis

The paper quantifies the spatial activity of a Drosophila enhancer through sequence mutagenesis and image analysis, revealing dense regulatory information and novel regulatory logic.

Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive demethylation in mammals

The paper identifies DPPA3 as a driver of genome-wide passive DNA demethylation in mammals, showing it can bypass TET protein requirements and function across species.

BigStitcher: Reconstructing high-resolution image datasets of cleared and expanded samples

BigStitcher reconstructs high-resolution image datasets from cleared and expanded biological samples, compensating optical distortions for single-cell analysis.

Heterochromatin drives organization of conventional and inverted nuclei

This paper investigates the mechanism of nuclear inversion in rod photoreceptors by combining Hi-C, microscopy, and polymer modeling, revealing that heterochromatin interactions drive phase separation and that lamina interactions maintain conventional nuclear organization.

Comparative analysis of single-cell RNA sequencing methods

This study compares five scRNA-seq protocols using mouse embryonic stem cells, finding Smart-seq2 most sensitive and SCRB-seq/Drop-seq most cost-efficient for differential gene detection.