This study identifies CDCA7 as a hemimethylated DNA sensor that interacts with HELLS to facilitate nucleosome remodeling and DNA methylation maintenance.

Science Advances · Aug 23, 2024Peer-reviewedMolecular Biology

The termination of UHRF1-dependent PAF15 ubiquitin signaling is regulated by USP7 and ATAD5

Ryota Miyashita, Atsuya Nishiyama, Weihua Qin, Yoshie Chiba, Satomi Kori, Norie Kato, Chieko Konishi, Soichiro Kumamoto, Hiroko Kozuka‐Hata, Masaaki Oyama, Yoshitaka Kawasoe, Toshiki Tsurimoto, Tatsuro S. Takahashi, Heinrich Leonhardt, Kyohei Arita, Makoto Nakanishi

This study identifies USP7 and ATAD5 as key factors in terminating UHRF1-dependent PAF15 ubiquitin signaling, linking this process to the completion of maintenance DNA methylation.

eLife · Feb 3, 2023Peer-reviewedMolecular Biology

HPF1-dependent PARP activation promotes LIG3-XRCC1-mediated backup pathway of Okazaki fragment ligation

Soichiro Kumamoto, Atsuya Nishiyama, Yoshie Chiba, Ryota Miyashita, Chieko Konishi, Yoshiaki Azuma, Makoto Nakanishi

The paper shows that PARP1-HPF1-dependent ADP-ribosylation recruits LIG3-XRCC1 to chromatin to mediate Okazaki fragment joining when LIG1 is absent.

Nucleic Acids Research · Apr 1, 2021Peer-reviewedMolecular Biology

Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive demethylation in mammals

Christopher B. Mulholland, Atsuya Nishiyama, Joël Ryan, Ryohei Nakamura, Merve Yiğit, Ivo M. Glück, Carina Trummer, Weihua Qin, Michael D. Bartoschek, Franziska R. Traube, Edris Parsa, Enes Ugur, Miha Modic, Aishwarya Acharya, Paul Stolz, , Heinrich Leonhardt

The paper identifies DPPA3 as a driver of genome-wide passive DNA demethylation in mammals, showing it can bypass TET protein requirements and function across species.

bioRxiv · Apr 4, 2020 · PreprintDevelopmental Biology