This study compares the effects of TREM2 R47H/+ and T66M mutations on iPSC-derived microglia, finding distinct gene expression and functional changes linked to neurodegeneration.

bioRxiv · Oct 5, 2022 · PreprintNeuroscience

Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration

Nader Morshed, William T. Ralvenius, Alexi Nott, Lauren Ashley Watson, Felicia H. Rodriguez, Leyla Anne Akay, Brian A. Joughin, Ping‐Chieh Pao, Jay Penney, Lauren M. LaRocque, Diego Mastroeni, Li‐Huei Tsai, Forest M. White

The paper uses phosphoproteomics to identify Siglec-F and Siglec-8 as upregulated in microglia during neurodegeneration, driving inflammatory responses in mouse and human models.

Molecular Systems Biology · Dec 1, 2020Peer-reviewedNeuroscience

Probing the functions of microglial cyclin-dependent kinase 5 under physiological and pathological conditions

Zhuyu Peng, Wen‐Chin Huang, Maggie Chen, Jay Penney, Hugh P. Cam, Fatema Abdurrob, Leyla Anne Akay, Xiao Chen, William T. Ralvenius, Lorena Pantano, Li‐Huei Tsai

This study investigates the role of Cdk5 in microglia under physiological and pathological conditions, finding transcriptional changes but minimal effects on morphology and behavior.

bioRxiv · May 12, 2020 · PreprintNeuroscience

Neuron Type-Specific Translatomes in Spinal Cords of Naive and Neuropathic Mice

Rebecca R Das Gupta, Louis Scheurer, Paweł Pelczar, William T. Ralvenius, Hendrik Wildner, Hanns Ulrich Zeilhofer

The paper uses TRAP to analyze translatomes of excitatory and inhibitory neurons in mouse spinal cords, finding neuropeptide genes associated with modality-specific processing and showing little translatome change in neuropathic pain.

bioRxiv · Mar 19, 2020 · PreprintNeuroscience