Ciaran M. Lee

Interrogating the Mechanisms of Cas9-mediated Allele Conversion

This paper develops a reporter system to study allele conversion mechanisms and identifies factors that enhance its efficiency.

Generation of Region-specific Airway Basal Stem Cells from Human Pluripotent Stem Cells via Regulation of NOGGIN-BMP Axis

This study generates region-specific airway basal stem cells from human pluripotent stem cells by modulating the NOGGIN-BMP axis, showing distinct differentiation potentials for proximal and distal airway cell types.

IL-32 drives inflammatory responses in IFN-γ primed human macrophages via a Myddosome-dependent pathway and is elevated in COVID-19

This study identifies IL-32β as a ligand that primes human macrophages for M1-like inflammatory responses via a Myddosome-dependent pathway and shows elevated IL-32 in severe COVID-19.

Haplotype rather than single causal variants effects contribute to regulatory gene expression associations in human myeloid cells

The paper validates the effects of SNPs on gene expression in myeloid cells and finds that haplotype effects, not single variants, explain many associations.

TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem Cells

The paper introduces TRACE-Seq, a method to track clonal reconstitution of gene-targeted human hematopoietic stem cells using barcoded AAV6 donor templates in immunodeficient mice.

Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I

This study uses CRISPR/Cas9 to edit hematopoietic stem cells to express iduronidase, correcting Mucopolysaccharidosis type I in mice.