Gang Bao

Proteome-wide quantification of protein turnover in frog and fly embryos reveals divergent strategies of maternal inheritance

The paper quantifies protein turnover in frog and fly embryos using 18O-water labeling and proteomics, revealing divergent strategies of maternal proteome inheritance.

High-Power Dual-Channel Chamber for High-Frequency Magnetic Neuromodulation

This paper introduces a dual-channel magnetic chamber for high-frequency magnetic neuromodulation in mice, capable of generating two independent magnetic fields with minimal interference and real-time behavioral monitoring.

Synergistic Arg-C Ultra and Lys-C Digestion for Quantitative Proteomics

This paper introduces a synergistic Lys-C/Arg-C Ultra digestion strategy to improve proteome coverage and quantitative reproducibility in shotgun proteomics.

In vivo expansion of gene-targeted hepatocytes through transient inhibition of an essential gene

This paper introduces a method to increase the proportion of correctly repaired hepatocytes in the liver through transient gene inhibition and transgene delivery, achieving a 25% increase in targeted cells and five-fold transgene expression.

Haplotype rather than single causal variants effects contribute to regulatory gene expression associations in human myeloid cells

The paper validates the effects of SNPs on gene expression in myeloid cells and finds that haplotype effects, not single variants, explain many associations.

Covalently linked adenovirus-AAV complexes as a novel platform technology for gene therapy

This paper introduces a novel gene therapy platform using covalently linked adenovirus-AAV complexes to improve co-infection efficiency and reduce AAV dose requirements.

The Need for Transfer Learning in CRISPR-Cas Off-Target Scoring

The paper develops a Siamese convolutional neural network for CRISPR-Cas off-target scoring and shows that transfer learning improves performance on a curated benchmark dataset.

TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem Cells

The paper introduces TRACE-Seq, a method to track clonal reconstitution of gene-targeted human hematopoietic stem cells using barcoded AAV6 donor templates in immunodeficient mice.

Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I

This study uses CRISPR/Cas9 to edit hematopoietic stem cells to express iduronidase, correcting Mucopolysaccharidosis type I in mice.