SYBR Gold staining of single stranded nucleic acids is strongly influenced by the presence of guanosines
Jakob Rien, Mauro Zimmermann, Jonathan Hall, Mihaela Zavolan, Nitish Mittal
The paper shows that SYBR Gold fluorescence is strongly influenced by guanosines in single-stranded nucleic acids, impacting its use in nucleic acid quantification.
bioRxiv · May 26, 2026 · PreprintMolecular Biology