John J Macklin

A novel rhodopsin-based voltage indicator for simultaneous two-photon optical recording with GCaMP in vivo

The paper introduces a novel rhodopsin-based voltage indicator, 2Photron, enabling simultaneous two-photon recording of spiking and subthreshold voltage with GCaMP in awake mice.

jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

The paper introduces jYCaMP1, a calcium indicator optimized for two-photon imaging at wavelengths above 1000 nm, showing improved performance in mice and flies.

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.

High-performance GFP-based calcium indicators for imaging activity in neuronal populations and microcompartments

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.

Sensitive red protein calcium indicators for imaging neural activity

The paper introduces improved red calcium indicators that match the sensitivity of GFP-based indicators, enabling better in vivo neural activity imaging.