Shaping cryogenic 3D volume imaging by pFIB ion species and milling voltage
Sanja Sviben, Christopher Thompson, Corinna Braun, Krishnan Venkataraman, Jessica Heebner, Ron Kelley, M. Zuhaib Qayyum, Abhay Kotecha, Ali Khalighifar, Georgios Skiniotis
This study investigates how ion species and milling voltage affect cryo-pFIB-SEM image quality, showing that optimal settings depend on specimen composition.
bioRxiv · Sep 20, 2026 · PreprintCell Biology