The paper demonstrates that a designed soluble Notch agonist can drive iAM maturation without odontoblast contact, revealing DLX3's role in ameloblast-specific Enamelin and MMP20 expression.

bioRxiv · Apr 3, 2025 · PreprintDevelopmental Biology

Human iPSC Derived Enamel Organoid Guided by Single-Cell Atlas of Human Tooth Development

Ammar Alghadeer, Sesha Hanson-Drury, Devon Duron Ehnes, Yan Ting Zhao, AP Patni, Diana R. O’Day, CH Spurrell, Aishwarya A. Gogate, Ashish Phal, H. Zhang, Arikketh Devi, Yuliang Wang, Lea M. Starita, Dan Doherty, Ian A. Glass, , Hannele Ruohola‐Baker

The paper establishes a single-cell atlas of human tooth development and uses it to guide the in vitro differentiation of ameloblasts from iPSCs, developing a mineralizing enamel organoid system.

bioRxiv · Aug 9, 2022 · PreprintDevelopmental Biology

Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions

Shiri Levy, Logeshwaran Somasundaram, Infencia Xavier Raj, Diego Ic-Mex, Sven Schmidt, Ammar Alghadeer, Henrik Honkanen, R. David Hawkins, Julie Mathieu, Yuliang Wang, David A. Baker, Karol Bomsztyk, Hannele Ruohola‐Baker

The paper uses a computationally designed PRC2 inhibitor, EBdCas9, to identify functional TATA boxes in distal promoter regions and demonstrate their role in gene activation.

bioRxiv · Nov 16, 2020 · PreprintMolecular Biology