Joshua D. Wythe

A combinatorial gene therapy targeting inflammation and chondrocyte health slows OA progression and reduces pain in murine and equine models.

This study shows that focused ultrasound with microbubbles opens the blood-tumor barrier in a glioma model, increasing CD4 and CD8 T cell infiltration without altering the CD8/Treg ratio.

This study identifies sensory fiber types in mouse TMJ tissues using immunohistochemistry, finding peptidergic fibers dominate with minimal non-peptidergic nociceptors.

The paper introduces KneEZ Clear, an aqueous-based tissue clearing method for musculoskeletal tissues that preserves fluorescence and avoids equipment requirements.

This study optimizes tissue clearing and imaging methods to achieve high-resolution 3D visualization of the vasculature in adult murine knee tissues.

The paper introduces MAGIC, a cloning system enabling modular assembly of gene constructs for use in vertebrate models.

This study shows that KRAS mutations drive brain arteriovenous malformations (bAVMs) in specific brain regions and that MEK inhibition can reduce their severity in mice, suggesting a potential therapeutic approach for human bAVMs.

EZ Clear provides a simple, rapid, and robust method for clearing whole adult mouse organs, preserving fluorescence and enabling downstream analysis.

This study identifies transcriptional and epigenetic mechanisms driving endothelial diversity across organs in mice, revealing conserved gene regulatory networks in the brain vasculature.

This study identifies S1PR1 as a regulator of lymphatic vessel quiescence by inhibiting LSS-dependent VEGF-C signaling in lymphatic endothelial cells.

The paper shows that Vegfa from alveolar type 1 cells specifies a distinct endothelial population in the mouse lung, marked by Car4 and involved in alveologenesis.

FishNET is an open-source relational database for managing zebrafish colony data, offering automated data entry, flexible queries, and integration of biological samples and records.

The paper develops a novel Dll4 reporter allele to accurately monitor Dll4 expression in embryonic and adult mice, overcoming limitations of previous reporter lines.