Toyoaki Natsume

Human FACT complex coordinates cohesin and transcription on chromatin

This study shows that the FACT complex regulates cohesin dynamics on chromatin, facilitating loop extrusion and preventing cohesin accumulation in transcribed regions.

Cohesin acts as a transcriptional gatekeeper by restraining pause-release to promote processive elongation

This study shows cohesin regulates multiple transcription steps, with loss leading to reduced induction but minimal steady-state expression changes.

Cohesin regulates promoter-proximal pausing of RNA Polymerase II by limiting recruitment of super elongation complex

This study shows cohesin limits super elongation complex recruitment to promoters, thereby regulating RNA Polymerase II pausing and gene expression.

Global landscape of replicative DNA polymerase usage in the human genome

The paper uses Pu-seq to map DNA polymerase usage in human cells, revealing Polε synthesizes the leading strand and Pol contributes to lagging strand synthesis, and links transcription to polymerase uncoupling and chromosomal instability.

Replication timing maintains the global epigenetic state in human cells.

This study shows that replication timing (RT) is essential for maintaining the global epigenetic state and genome compartmentalization in human cells, with RIF1 playing a central role in this process.

RIF1-Long promotes G1 phase 53BP1 nuclear bodies to protect against replication stress

This study identifies RIF1-Long's role in promoting G1 phase 53BP1 nuclear bodies to protect against replication stress and enhance cell survival.