Shigeki Kiyonaka

Off-target-free chemogenetic platform that decodes physiological roles of target GPCRs

The paper introduces an off-target-free chemogenetic platform to study GPCR functions, demonstrating its application on the adenosine A2A receptor and showing its generalizability to other class A GPCRs.

Spatial ciliary signaling regulates the dorsal/ventral regionalization of human brain organoids

Native receptor-targeted chemogenetics enables cell-type-specific inhibition of endogenous receptors in freely moving mice

This paper introduces NARCH, a chemogenetic method to reversibly inhibit endogenous receptors in specific cell types in vivo, demonstrating its use in studying mGlu1 signaling in Purkinje cells.

A fluorophore-ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

This paper introduces a chemical probe for visualizing AMPA receptors in living neurons without genetic modification, revealing synaptic plasticity mechanisms.

Synaptic Organization of Surface AMPARs Changes by Brain Region and Tauopathy

This study maps native surface AMPAR organization in mouse brain regions and tauopathy models using dSTORM imaging, revealing regional differences and early synaptic remodeling in tauopathy.

Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue

This paper develops a chemogenetic method to selectively activate mGlu1 receptors in brain tissue using a palladium complex and a mutant receptor, demonstrating its role in synaptic plasticity.

Revisiting PFA-mediated tissue fixation chemistry: FixEL enables trapping of small molecules in the brain to visualize their distribution dynamics

The paper introduces FixEL, a method to trap and image small molecules in brain tissue, enabling visualization of their distribution and dynamics.