EnrichSci enables targeted profiling of specific cell types in complex tissues, revealing aging-associated molecular dynamics and post-transcriptional regulation.

bioRxiv · May 2, 2025 · PreprintGenomics

Uncovering Convergent Cell State Dynamics Across Divergent Genetic Perturbations Through Single-Cell High-Content CRISPR Screening

Zihan Xu, Ziyu Lu, Aileen Ugurbil, Abdulraouf Abdulraouf, Andrew Liao, Jianxiang Zhang, Wei You Zhou, Junyue Cao

The paper introduces PerturbFate, a single-cell CRISPR screening platform, and finds convergent cell state dynamics across diverse genetic perturbations in melanoma cells.

bioRxiv · May 2, 2025 · PreprintGenomics

High resolution deep mutational scanning of the melanocortin-4 receptor enables target characterization for drug discovery

Conor J Howard, Nathan S. Abell, Beatriz A. Osuna, Eric M. Jones, Leon Y Chan, Henry W.-S. Chan, Dean R. Artis, Jonathan B Asfaha, Joshua S. Bloom, Aaron R. Cooper, Andrew Liao, Eden Mahdavi, Nabil Mohammed, Alan L Su, Giselle A. Uribe, , Nathan B. Lubock

This study applies high-resolution deep mutational scanning to the melanocortin-4 receptor to identify functionally significant variants and guide drug development.

eLife · Mar 26, 2025Peer-reviewedSynthetic Biology

A global view of aging and Alzheimer's pathogenesis-associated cell population dynamics and molecular signatures in the human and mouse brains

Andras Sziraki, Ziyu Lu, Jasper Lee, Gábor Bányai, Sonya Anderson, Abdulraouf Abdulraouf, Eli Metzner, Andrew Shuh-Huei Liao, Jason R. Banfelder, Alexander E. Epstein, Chloe Schaefer, Zihan Xu, Zehao Zhang, Li Gan, Peter T. Nelson, , Junyue Cao

The paper develops a high-throughput single-cell genomics approach to study age-related and Alzheimer's disease-associated cell population dynamics and molecular signatures in human and mouse brains.

Nature Genetics · Nov 30, 2023Peer-reviewedGenomics