Michael Lisby

CRI-SPA, a mating based CRISPR-Cas9 assisted method for high-throughput genetic modification of yeast strain libraries

CRI-SPA transfers genetic features between yeast strains to map sequence to function, demonstrating its ability to transfer genes for betaxanthin production across a yeast knock-out collection.

Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes.

This study shows that Top2 deficiency in yeast causes a structural shift in chromosomes after replication, leading to segregation issues without triggering G2/M checkpoints.

Folliculin variants linked to Birt-Hogg-Dube syndrome are targeted for proteasomal degradation

The paper shows that BHD-linked FLCN variants cause loss of function via proteasomal degradation and misfolding, with potential therapeutic implications for stabilization.

A critical role for Dna2 at unwound telomeres

The paper identifies Dna2's critical role at telomeres by showing its involvement in telomere length regulation and DNA damage response modulation through nuclease activity.

Genome-scale genetic interactions position the Mitotic Exit Network as a major antagonist of transient Topoisomerase II deficiency.

The paper identifies the Mitotic Exit Network as a protective factor against Top2 deficiency by comparing yeast strains with different Top2 alleles.