Yuhei Goto

Opto-MDMi: a dual-lock optogenetic system for robust activation of endogenous p53

This paper introduces Opto-MDMi, a dual-lock optogenetic system that activates endogenous p53 with light, while suppressing basal activity in the dark.

Regulation of cell proliferation by a novel feedback system on Cdk function

This study identifies a novel feedback system regulating Cdk function through Spo12, a small disordered protein that inhibits PP2A, and shows its role in enhancing cell proliferation in fission yeast.

Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins.

This study measures cyclin-CDK dissociation constants in living cells using FCCS with optimized fluorescent proteins, revealing insights into cyclin-CDK binding redundancy.

Opto-p53: A Light-Controllable p53 Signaling for Regulating p53-Dependent Cell Fate.

This study develops Opto-p53, a light-controllable p53 signaling tool, to regulate p53-dependent cell fate by inducing transcriptional activation, cell death, and cell cycle arrest.

Network topology creates independent control of G2-M from G1-S checkpoints in the fission yeast cell cycle system

The paper uses a topology-based theory to predict and validate independent control of G2-M and G1-S checkpoints in fission yeast cell cycle by analyzing CDK-cyclin complex dynamics.

Chemogenetic manipulation of endogenous proteins in fission yeast using a self-localizing ligand-induced protein translocation (SLIPT) system

This study applies the SLIPT system to fission yeast to perturb cell cycle-related proteins with improved temporal resolution.

Improvement of phycocyanobilin synthesis for genetically encoded phytochrome-based optogenetics.

The paper improves a PCB synthesis system for a PhyB-PIF optogenetic tool, enabling stable expression and light-inducible dimerization in cells.