Susanne tom Dieck

Brain-wide synaptosome profiling reveals localized mRNAs that diversify synapses

The paper profiles synaptic transcriptomes across five brain regions in mice, identifying mRNAs enriched at synapses and their role in synaptic diversity and function.

Cell type-Specific In Vivo Proteomes with a Multi-copy Mutant Methionyl t-RNA Synthetase Mouse Line

This paper introduces a multicopy MetRS* mouse line to enhance cell type-specific in vivo protein labeling, enabling sensitive detection of newly synthesized proteins in sparse cell populations.

Neuronal processes contain the essential components for the late steps of ribosome biogenesis

The paper shows that ribosome biogenesis factors and pre-rRNA processing enzymes are present in distal neuronal compartments, suggesting local ribosome maturation occurs in dendrites.

The proteomic landscape of synaptic diversity across brain regions and cell types

The paper identifies synapse type-enriched proteins and their functional associations across brain regions and cell types using proteomic analysis.

Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

The paper develops a method to profile subcellular transcriptomes of single neurons, revealing that GABAergic interneurons have localized mRNAs similar to excitatory neurons.

Surfaceome dynamics during neuronal development and synaptic plasticity reveal system-wide surfaceome reorganization independent of global proteostasis

The paper analyzes surfaceome dynamics during neuronal development and synaptic plasticity, revealing surface protein reorganization independent of global proteostasis.

The mRNA translation landscape in the synaptic neuropil.

The paper identifies and quantifies the transcriptome and translatome in neuronal cell bodies, dendrites, and axons using RNA-seq and Ribo-seq, revealing that local translation is a major source of synaptic proteins.

Neuronal Proteostasis is mediated by the switch-like expression of Heme-regulated Kinase 1, acting as both a sensor and effector

Proteasome inhibition leads to reduced neuronal protein synthesis through stabilization and enhanced translation of HRI kinase, which is constitutively active due to low neuronal heme levels.