Julian D. Langer

Breaking new ground into RAD51-BRC repeats interplay in Homologous Recombination

This study investigates the RAD51-BRC repeats interaction using computational and experimental methods to better understand its role in homologous recombination and its implications for cancer.

Mouse Neural Organoids Model the Mature In Vivo Synaptic Proteome

The paper shows that mouse neural organoids can replicate the synaptic proteome of mature brains through optimized protocols and proteomics analysis.

Ubiquitin selective ribosome profiling reveals systematic principles of co-translational quality control

The paper introduces UbSeRP to map co-translational ubiquitination across the translatome, revealing RQC substrates, biophysical features predicting RQC, and aging-related changes in CTU.

SUMO paralogues differentially affect phase separation and aggregation of intrinsically disordered proteins

This study shows SUMO1 and SUMO2 differentially regulate TDP-43 phase separation and aggregation, with SUMO1 inhibiting and SUMO2 promoting liquid-like states that prevent aggregation.

The vault associates with membranes in situ

This study uses cryo-electron tomography to show vault particles associate with ER and nuclear envelope membranes and enclose ribosomes in Dictyostelium discoideum.

Direct interaction of ribosomes with postsynaptic proteins gives rise to a privileged local synaptic translatome

The paper identifies a direct interaction between postsynaptic proteins and ribosomes, revealing a localized synaptic translatome that preferentially translates PSD-related mRNAs.

Cathepsin-dependent amyloid formation drives mechanical rupture of lysosomal membranes

This study shows that LLOMe forms amyloid structures inside lysosomes that mechanically rupture the lysosomal membrane, revealing a structural mechanism for lysosomal damage.

Correlative MS Imaging for in situ cryo-ET

This paper introduces a combined MSI and cryo-ET pipeline for reliable single-cell classification in cryo-ET samples, enabling post-acquisition cell identification and classification.

The molecular diversity of hippocampal regions and strata at synaptic resolution revealed by integrated transcriptomic and proteomic profiling

The paper integrates transcriptomic and proteomic data from mouse hippocampal regions and synapses to reveal molecular diversity and co-regulation patterns.

Cell type-Specific In Vivo Proteomes with a Multi-copy Mutant Methionyl t-RNA Synthetase Mouse Line

This paper introduces a multicopy MetRS* mouse line to enhance cell type-specific in vivo protein labeling, enabling sensitive detection of newly synthesized proteins in sparse cell populations.

Neuronal processes contain the essential components for the late steps of ribosome biogenesis

The paper shows that ribosome biogenesis factors and pre-rRNA processing enzymes are present in distal neuronal compartments, suggesting local ribosome maturation occurs in dendrites.

SND3 is the membrane insertase within a fungal multipass translocon

This study identifies SND3 as a novel membrane insertase in the fungal SND pathway and presents its structure and mechanism for inserting multipass integral membrane proteins.

Structural insights into late-stage photosystem II assembly by Psb32

This study determines the structure of two late-stage PSII assembly intermediates, revealing how Psb32 and Psb27 regulate OEC maturation and Mn4O5Ca cluster preparation.

The small membrane protein YohP induces membrane depolarization and ppGpp accumulation in Escherichia coli.

This study identifies YohP as a small membrane protein in E. coli that induces membrane depolarization and (p)ppGpp accumulation, leading to metabolic silencing under stress.

The proteasome maturation factor POMP moonlights as a stress-induced transcriptional regulator

This study identifies POMP as a stress-induced transcriptional regulator that buffers proteasome dysfunction through a non-canonical role in nucleolar RNA processing.

Integrating computational and experimental biophysics reveals novel insights into the RAD51-BRC4 interaction

This study combines computational and experimental methods to characterize the conformational dynamics of the RAD51-BRC4 complex, revealing key residues involved in their interaction.

p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partners

VAP spatially stabilizes dendritic mitochondria to locally fuel synaptic plasticity

The paper identifies VAP as a protein that stabilizes mitochondria near synapses to support synaptic plasticity and determines the spatial extent of dendritic segments involved.

The proteomic landscape of synaptic diversity across brain regions and cell types

The paper identifies synapse type-enriched proteins and their functional associations across brain regions and cell types using proteomic analysis.

How does Mycoplasma pneumoniae steal lipids from its host membranes?

This study identifies how Mycoplasma pneumoniae uses the protein P116 to transport lipids between membranes without ATP, revealing a simplified lipid uptake mechanism.

An Abundance of Free Proteasomal Regulatory (19S) Particles Regulate Neuronal Synapses Independent of the Proteasome

The paper identifies a proteasomal 19S subcomplex that regulates neuronal synapses independently of the 20S catalytic subunit through deubiquitylation of Lys63-ubiquitin.

Co-translational assembly counteracts promiscuous interactions

The paper identifies co-translational assembly domains in proteins that facilitate interactions between nascent chains and fully synthesized subunits, revealing their role in regulating complex assembly pathways.

Neuronal ribosomes dynamically exchange ribosomal proteins in a context-dependent manner.

The paper shows that neuronal ribosomal proteins dynamically exchange in a context-dependent manner, independent of canonical ribosome biogenesis.

Dynamic bi-directional phosphorylation events associated with the reciprocal regulation of synapses during homeostatic up- and down-scaling.

The paper identifies dynamic phosphorylation events during homeostatic synaptic scaling and finds reciprocal regulation of phosphoproteins in up- and down-scaling.

Monosomes actively translate synaptic mRNAs in neuronal processes

The paper shows that monosomes, not polysomes, are the main sites of active translation for synaptic mRNAs in neuronal processes, contributing to local protein synthesis.