Erin Margaret Schuman

Mouse Neural Organoids Model the Mature In Vivo Synaptic Proteome

The paper shows that mouse neural organoids can replicate the synaptic proteome of mature brains through optimized protocols and proteomics analysis.

Localized mRNAs and protein synthesis in cortical layer 1

The paper identifies localized mRNAs and proteins in cortical layer 1 synapses, showing specialized transcriptomes and suggesting local translation supports layer-specific functions.

Local translation drives glioblastoma heterogeneity and tumor invasion

This study identifies local translation as a driver of glioblastoma tumor microtubes and invasion by linking subcellular protein synthesis to invasive cell states.

Direct interaction of ribosomes with postsynaptic proteins gives rise to a privileged local synaptic translatome

The paper identifies a direct interaction between postsynaptic proteins and ribosomes, revealing a localized synaptic translatome that preferentially translates PSD-related mRNAs.

Cathepsin-dependent amyloid formation drives mechanical rupture of lysosomal membranes

This study shows that LLOMe forms amyloid structures inside lysosomes that mechanically rupture the lysosomal membrane, revealing a structural mechanism for lysosomal damage.

Brain-wide synaptosome profiling reveals localized mRNAs that diversify synapses

The paper profiles synaptic transcriptomes across five brain regions in mice, identifying mRNAs enriched at synapses and their role in synaptic diversity and function.

Correlative MS Imaging for in situ cryo-ET

This paper introduces a combined MSI and cryo-ET pipeline for reliable single-cell classification in cryo-ET samples, enabling post-acquisition cell identification and classification.

The molecular diversity of hippocampal regions and strata at synaptic resolution revealed by integrated transcriptomic and proteomic profiling

The paper integrates transcriptomic and proteomic data from mouse hippocampal regions and synapses to reveal molecular diversity and co-regulation patterns.

Cell type-Specific In Vivo Proteomes with a Multi-copy Mutant Methionyl t-RNA Synthetase Mouse Line

This paper introduces a multicopy MetRS* mouse line to enhance cell type-specific in vivo protein labeling, enabling sensitive detection of newly synthesized proteins in sparse cell populations.

Neuronal processes contain the essential components for the late steps of ribosome biogenesis

The paper shows that ribosome biogenesis factors and pre-rRNA processing enzymes are present in distal neuronal compartments, suggesting local ribosome maturation occurs in dendrites.

The proteasome maturation factor POMP moonlights as a stress-induced transcriptional regulator

This study identifies POMP as a stress-induced transcriptional regulator that buffers proteasome dysfunction through a non-canonical role in nucleolar RNA processing.

An integrative model of AMPA receptor trafficking reveals the central contribution of local translation in subtype-specific kinetics

The paper develops a computational model to explain how local translation of CNIH-2 affects the trafficking and kinetics of different AMPAR subtypes during long-term potentiation.

The proteomic landscape of synaptic diversity across brain regions and cell types

The paper identifies synapse type-enriched proteins and their functional associations across brain regions and cell types using proteomic analysis.

An Abundance of Free Proteasomal Regulatory (19S) Particles Regulate Neuronal Synapses Independent of the Proteasome

The paper identifies a proteasomal 19S subcomplex that regulates neuronal synapses independently of the 20S catalytic subunit through deubiquitylation of Lys63-ubiquitin.

Control of neocortical memory by long-range inhibition in layer 1

The paper identifies long-range inhibitory input to layer 1 of the auditory cortex and shows its role in facilitating memory through plasticity during threat learning.

Unbiased choice of global clustering parameters in single-molecule localization microscopy

This paper introduces an unbiased clustering algorithm for single-molecule localization microscopy that reduces false positives and negatives compared to existing methods.

Oxytocin receptors influence the development and maintenance of social behavior in zebrafish (Danio rerio).

This study shows that oxytocin receptors influence the development and maintenance of social behavior in zebrafish, with effects varying by age and social context.

Co-translational assembly counteracts promiscuous interactions

The paper identifies co-translational assembly domains in proteins that facilitate interactions between nascent chains and fully synthesized subunits, revealing their role in regulating complex assembly pathways.

The neuropeptide Pth2 modulates social behavior and anxiety in zebrafish

This study shows that the neuropeptide Pth2 in zebrafish affects anxiety and social behavior, with altered responses to stimuli and changes in social preference during development.

Neuronal ribosomes dynamically exchange ribosomal proteins in a context-dependent manner.

The paper shows that neuronal ribosomal proteins dynamically exchange in a context-dependent manner, independent of canonical ribosome biogenesis.

The Spatial Scale of Synaptic Protein Allocation during Homeostatic Plasticity

The paper quantifies the spatial scale of synaptic protein allocation during homeostatic plasticity and finds that proteins are distributed at the level of synaptic neighborhoods.

Dynamic bi-directional phosphorylation events associated with the reciprocal regulation of synapses during homeostatic up- and down-scaling.

The paper identifies dynamic phosphorylation events during homeostatic synaptic scaling and finds reciprocal regulation of phosphoproteins in up- and down-scaling.

Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression

The paper investigates how long-term potentiation and depression differentially regulate mRNA dynamics and translation in neurons.

Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

The paper develops a method to profile subcellular transcriptomes of single neurons, revealing that GABAergic interneurons have localized mRNAs similar to excitatory neurons.

The mRNA translation landscape in the synaptic neuropil.

The paper identifies and quantifies the transcriptome and translatome in neuronal cell bodies, dendrites, and axons using RNA-seq and Ribo-seq, revealing that local translation is a major source of synaptic proteins.

Monosomes actively translate synaptic mRNAs in neuronal processes

The paper shows that monosomes, not polysomes, are the main sites of active translation for synaptic mRNAs in neuronal processes, contributing to local protein synthesis.

Neuronal Proteostasis is mediated by the switch-like expression of Heme-regulated Kinase 1, acting as both a sensor and effector

Proteasome inhibition leads to reduced neuronal protein synthesis through stabilization and enhanced translation of HRI kinase, which is constitutively active due to low neuronal heme levels.

Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments.

The paper demonstrates that local protein synthesis occurs in both pre- and postsynaptic compartments of neurons, including excitatory and inhibitory axon terminals.

Poly(A) Binding Protein Nuclear 1 regulates the polyadenylation of key synaptic plasticity genes and plays a role in homeostatic plasticity

This study shows that Pabpn1 regulates polyadenylation of synaptic plasticity genes and affects homeostatic plasticity in neurons.