Abhishek Chatterjee

This paper describes a chemical strategy to create AAV-protein conjugates for targeted gene delivery, showing efficient and selective transduction of HER2+ cancer cells with minimal off-target effects.

This study optimizes a yeast-based directed evolution platform to expand the substrate scope of EcLeuRS, enabling incorporation of noncanonical amino acids like ornithine into mammalian cells.

This paper improves the efficiency of noncanonical amino acid incorporation in mammalian cells by evolving tRNAEcLeu through directed evolution in mammalian cells.

This paper introduces a translation-independent method to engineer aminoacyl-tRNA synthetases that can incorporate noncanonical amino acids into proteins.

This study shows that precise control over the site and stoichiometry of capsid modification in AAV vectors improves retargeting efficiency and activity.

This paper introduces a method to synthesize proteins with internal β2-peptide linkages in cells by using a post-translational rearrangement after incorporating specific -hydroxy acid monomers.

This paper introduces an efficient method to incorporate up to three distinct noncanonical amino acids into proteins in mammalian cells using a combination of orthogonal aaRS/tRNA pairs.

This paper develops VADER, a virus-assisted directed evolution method for improving suppressor tRNAs in mammalian cells, resulting in more active and orthogonal tRNA mutants.

This paper describes an engineered E. coli tyrosyl-tRNA synthetase system that enables the incorporation of new noncanonical amino acids into eukaryotic cells.

This paper demonstrates that the engineered organism Vmax X2 enables high-yield and high-fidelity incorporation of non-canonical amino acids into proteins.

The paper shows that structural robustness of tyrosyl-tRNA synthetases affects their ability to be engineered for genetic code expansion, with MjTyrRS being more robust than EcTyrRS.