Rotem Karni

Targeting the 3' splice site by a decoy oligonucleotide attenuates U2AF1 splicing activity and inhibits leukemia

This paper develops an RNA decoy strategy to inhibit splicing factor activity in leukemia, showing it reduces tumor burden in vivo regardless of spliceosomal mutations.

Allele-specific antisense oligonucleotide treatment rescues atad3-associated phenotype in zebrafish

This study demonstrates that allele-specific antisense oligonucleotides can rescue ATAD3A-associated developmental defects in zebrafish by reducing mutant transcripts.

FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN)

This study shows FTO regulates centrosome function and mitotic fidelity, with CIN cancers being more sensitive to FTO inhibition.

Molecular characterization of the heterozygous loss of function mutations in the X-linked PCDH19 gene causing PCDH19-Cluster Epilepsy

The paper investigates PCDH19-Cluster Epilepsy by generating mouse and human models, identifying differentially expressed genes and morphological changes linked to PCDH19 mutations.

RNA splicing dynamics in CD8 T cells uncovers isoforms that impact T cell-mediated cancer immunotherapy

The paper constructs a single-cell splicing atlas of human CD8 T cells and identifies isoform-specific immune checkpoints that enhance effector function and tumor control.

The prevalent NR2E3 c.932G>A mutation induces aberrant splicing that can be rescued using splice-shifting antisense oligonucleotides

This study identifies aberrant splicing caused by the NR2E3 c.932G>A mutation and shows that splice-shifting ASOs can restore full-length protein function.