Michael S. Westphall

Fast and Deep Phosphoproteome Analysis with the Orbitrap Astral Mass Spectrometer

The paper introduces a method using the Orbitrap Astral mass spectrometer with DIA to rapidly analyze phosphoproteomes, detecting over 80,000 phosphorylation sites in mice.

Boosting the Sensitivity of Quantitative Single-Cell Proteomics with Activated Ion-Tandem Mass Tags (AI-TMT)

This paper introduces AI-TMT, a method enhancing single-cell proteomics sensitivity by improving reporter ion generation, resulting in higher signal and faster analysis.

3D Structure Determination of Protein Complexes using Matrix-Landing Mass Spectrometry

This paper introduces a modified mass spectrometry system for depositing gas-phase protein complexes onto EM grids, enabling 3D structure determination of non-covalent protein complexes like GroEL.

Proteomics, lipidomics, metabolomics and 16S rDNA sequencing of dental plaque from patients with diabetes and periodontal disease

The paper integrates proteomics, lipidomics, metabolomics, and 16S rDNA sequencing to analyze dental plaque from patients with diabetes and periodontal disease, identifying microbial and host factors associated with these conditions.

Mapping physiological ADP-ribosylation using Activated Ion Electron Transfer Dissociation (AI-ETD)

The paper introduces AI-ETD for MS-based proteomics analysis of physiological ADP-ribosylation, identifying more ADPr sites than existing methods.

Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis

This study captures site-specific N-glycoproteome heterogeneity using AI-ETD, identifying 1,545 N-glycosites from mouse brain tissue and developing new visualizations for glycopeptide analysis.