Olivier R. Thoumine

Adaptive-optics lattice light-sheet microscopy enables correlative single-molecule imaging of endogenous proteins in native environments

ALPINE enables 3D single-molecule imaging and tracking of endogenous proteins in native environments with high resolution and dynamic quantification.

Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses

This paper introduces a 3D neurosphere model from rodent brain cells to study synapse structure and function using advanced imaging and electrophysiology.

A nanobody-gold conjugate for the detection of GFP-tagged synaptic proteins by dual light-electron microscopy

The paper develops a nanobody-gold conjugate for dual light-electron microscopy to label GFP-tagged synaptic proteins with high specificity and nanoscale resolution.

High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain

This study develops a tagged knock-in mouse strain to visualize and isolate endogenous neuroligin-1, revealing its presence at both excitatory and inhibitory synapses.

Dissecting the binding mechanisms of synaptic membrane adhesion complexes using a micropattern based cellular model

This study measures the binding kinetics of synaptic ligand-receptor interactions using a micropatterned cellular model and identifies differences in binding regimes between SynCAM1 and Nrx1β-Nlg1 complexes.

MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior

The paper shows MDGAs delay excitatory synapse development by altering neuroligin behavior through fast diffusion and reduced mobility.

mir124-dependent tagging of glutamatergic synapses by synaptopodin controls non-uniform and input-specific homeostatic synaptic plasticity

The paper identifies synaptopodin as a synaptic tag regulated by miR124 that enables non-uniform and input-specific homeostatic synaptic plasticity in response to activity deprivation.

Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation

This study uses optogenetics to show that phosphorylation of neuroligin-1 promotes excitatory synaptic differentiation and potentiation.

Iterative inhibition of commissural growth cone exploration, not post-crossing barrier, ensures forward midline navigation through SlitC-PlxnA1 signaling

The paper shows that SlitC-PlxnA1 signaling limits growth cone exploration to ensure forward midline navigation, not post-crossing barrier mechanisms.

Spatial and temporal profiling of receptor membrane insertion controls commissural axon responses to midline repellents

The paper investigates how commissural axons develop sensitivity to midline repellents through receptor dynamics during navigation, revealing spatial and temporal receptor profiles that enable discrimination of repulsive signals.

Pre-post synaptic alignment through neuroligin tunes synaptic transmission efficiency

The paper shows that neuroligin-1 alignment with AMPA receptor nanodomains is critical for synaptic transmission efficiency, as disrupting this alignment reduces transmission.