Charles Ducrot

Hindbrain explants enable multimodal and longitudinal analysis of the developing olivo-cerebellar circuit at single-cell resolution

This paper introduces an in vitro model of embryonic mouse hindbrain explants that enables longitudinal, multimodal analysis of olivo-cerebellar circuit development at single-cell resolution.

Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses

This paper introduces a 3D neurosphere model from rodent brain cells to study synapse structure and function using advanced imaging and electrophysiology.

Loss of neurexins disrupts inhibitory connectivity and increases vulnerability of dopamine neurons in culture

This study shows that loss of neurexins in dopamine neurons disrupts inhibitory synapse formation and reduces neuronal survival in culture.

A nanobody-gold conjugate for the detection of GFP-tagged synaptic proteins by dual light-electron microscopy

The paper develops a nanobody-gold conjugate for dual light-electron microscopy to label GFP-tagged synaptic proteins with high specificity and nanoscale resolution.

High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain

This study develops a tagged knock-in mouse strain to visualize and isolate endogenous neuroligin-1, revealing its presence at both excitatory and inhibitory synapses.

Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.

This study shows that phasic dopamine release in mice is not essential for basic motor behaviors, as motor functions remain intact despite near-complete loss of activity-dependent dopamine release.

Neurexins Regulate GABA Co-release by Dopamine Neurons

This study shows that neurexins regulate dopamine neuron connectivity and neurotransmission by affecting dopamine reuptake and GABA co-release.

Neonatal 6-OHDA lesion of the SNc induces striatal compensatory sprouting from surviving SNc dopaminergic neurons without VTA contribution

This paper shows that neonatal 6-OHDA lesions of the SNc in mice lead to compensatory axonal sprouting from surviving SNc dopaminergic neurons, without contribution from VTA neurons.

Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals

This study shows dopaminergic neurons form a unique axonal arbor with mostly non-synaptic terminals, expressing exocytosis proteins and being active, and highlights the role of specific proteins in synapse formation.

Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release

The paper investigates somatodendritic dopamine release in mice, showing that synaptotagmin 4 and 7 are required for this process, while axonal release remains unaffected.