Paolo Ronchi

Microtubules sustain the fidelity of cellularization in a coenocytic relative of animals

This study investigates cellularization in Sphaeroforma arctica, showing microtubules help guide plasma membrane furrows to ensure equal partitioning of nuclei and cytoplasm during division.

Workflow and instrumentation for multiscale correlative imaging in voluminous cryogenic specimens

This paper introduces a multiscale correlative imaging workflow for cryogenic specimens, enabling fluorescence-guided site preparation and targeted lamellae lift-out for cryo-ET.

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis

The paper shows hydrophobic FG-nucleoporins dilate nuclear pores during mitosis, enabling nuclear import and NE reformation.

iSBEM: An Open-Source Workflow for Automated ROI Targeting in Volume Electron Microscopy

iSBEM automates ROI targeting in SBF-SEM using XRM maps, improving acquisition throughput while maintaining targeting precision in biological specimens.

Mesoglea biogenesis reveals a cryptic aboral valve for pressure regulation in cnidarian morphogenesis

The paper identifies a pressure-sensitive aboral valve in cnidarians that regulates hydraulic pressure during morphogenesis through mesogleal basement membrane dynamics.

RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion

The paper identifies RanBP2-dependent annulate lamellae as a distinct pathway for nuclear pore assembly and nuclear expansion in somatic cells.

An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales

The paper introduces an Advanced Mobile Laboratory (AML) for field-based microbial ecology and cell biology, enabling multiscale analysis of environmental samples directly at the sampling site.

Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model

This paper shows that Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model, leading to vascular dysfunction that can be partially reversed with Ang-1 or AKB-9778.

The environmental stress response controls the biophysical properties of the cytoplasm and is critical for survival in quiescence

This study shows that respiration-induced gene expression and stress response pathways are critical for maintaining cytoplasmic properties and survival in yeast quiescence.

In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography

This study uses cryo-ET to visualize copia retrotransposon structure in Drosophila cells, revealing nuclear clustering and size selection during gametogenesis.

BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the mitochondrial complex III

The paper identifies fedratinib as a drug that increases endoplasmic reticulum-mitochondria contact sites by inhibiting BRD4, revealing a novel epigenetic pathway linking ERMCS to cellular metabolism.

The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus

This study identifies the BASELESS gene as encoding a PP2A regulatory subunit essential for initial cell division and basal cell fate establishment in Ectocarpus.

Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy

The paper demonstrates that dAPEX2/DAB labeling is compatible with 3D volume EM techniques to map long-range synaptic inputs in the mouse brain.

Profiling cellular diversity in sponges informs animal cell type and nervous system evolution

The paper identifies 18 cell types in sponges, including neuroid cells with synaptic gene expression, suggesting early neuro-immune system evolution.

Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells

This study shows that tunneling nanotubes (TNTs) mediate stroma-mediated imatinib resistance in leukemic cells by transferring survival proteins.