This paper develops excitation ratiometric fluorescent protein biosensors for citrate, enabling quantitative analysis of citrate dynamics in different cellular compartments and populations.

bioRxiv · Apr 20, 2026 · PreprintMolecular Biology

Metabolic Crossroads: AMDHD2 Couples the Hexosamine Biosynthetic Pathway to Acetyl-CoA Homeostasis in Pluripotent Stem Cells

Virginia Kroef, Lea Hund, Yasmin Barenco Abbas, Norito Tamura, Jenny Fröhlich, Ann‐Kathrin Huber, Syed Musa Ali, Lena Wischhof, Hannah Scheiblich, Patrick Giavalisco, Martin Sebastian Denzel, Peter Tessarz, Ina Huppertz

This study identifies AMDHD2 as a key regulator linking the hexosamine biosynthetic pathway to acetyl-CoA homeostasis in mouse embryonic stem cells, revealing a metabolic mechanism critical for differentiation.

bioRxiv · Aug 20, 2025 · PreprintMolecular Biology

The RNA-binding protein landscapes differ between mammalian organs and cultured cells

Joel I. Perez-Perri, Dunja Ferring–Appel, Ina Huppertz, Thomas Schwarzl, Sudeep Sahadevan, Frank Stein, Mandy Rettel, Bruno Galy, Matthias W. Hentze

The paper characterizes RNA-binding protein landscapes in mouse organs using ex vivo eRIC, identifying over 1300 RBPs, many not previously found in cultured cells.

Nature Communications · Apr 12, 2023Peer-reviewedMolecular Biology

RNA regulates Glycolysis and Embryonic Stem Cell Differentiation via Enolase 1

Ina Huppertz, Joel I. Perez-Perri, Panagiotis Mantas, Thileepan Sekaran, Thomas Schwarzl, Francesco Russo, Dunja Ferring–Appel, Zuzana Koskova, Lyudmila Dimitrova-Paternoga, Eleni Kafkia, Janosch Hennig, Pierre A. Neveu, Kiran Raosaheb Patil, Matthias W. Hentze

The paper shows RNA regulates ENO1 enzyme activity, affecting glycolysis and stem cell differentiation in human and mouse cells.

Molecular Cell · Jun 15, 2022Peer-reviewedMolecular Biology