This paper determines the structure of the cyanobacterial NDH-1MS complex using cryo-EM, revealing a revised model for CO2 hydration coupled to proton transfer and electron flow.

bioRxiv · Aug 4, 2026 · PreprintPlant Biology

Structural Basis of Membrane Potential Coupled Vectorial CO2 Hydration by the DAB2 Complex in Chemolithoautotrophs

Yat Kei Lo, Michael Seletskiy, Stefan Bohn, Darja Deobald, Timo Glatter, Sven Timo Stripp, Jan M. Schuller

This paper presents the first cryo-EM structure of DAB2, revealing a PMF-driven CO2 hydration mechanism in chemolithoautotrophs.

Nature Communications · May 5, 2026Peer-reviewedBiochemistry

Structural insights into late-stage photosystem II assembly by Psb32

Stefan Bohn, Yat Kei Lo, Jan Lambertz, Jakob Meier-Credo, Torben Fürtges, Pasqual Liauw, Raphael Gasper-Schönenbrücher, Dennis Wiens, Julian D. Langer, Georg Hochberg, Eckhard Hofmann, Till Rudack, Marc M. Nowaczyk, Jan M. Schuller

This study determines the structure of two late-stage PSII assembly intermediates, revealing how Psb32 and Psb27 regulate OEC maturation and Mn4O5Ca cluster preparation.

bioRxiv · May 8, 2025 · PreprintPlant Biology

Frequent transitions in self-assembly across the evolution of a central metabolic enzyme

Franziska L. Sendker, Tabea Schlotthauer, Christopher-Nils Mais, Yat Kei Lo, Mathias Girbig, Stefan Bohn, Thomas Heimerl, Daniel Schindler, Arielle Weinstein, Brian P. H. Metzger, Joseph W. Thornton, Arvind S. Pillai, Gert Bange, Jan M. Schuller, Georg K. A. Hochberg

The paper investigates the evolutionary dynamics of self-assembly in prokaryotic citrate synthases, finding frequent transitions between different assembly types without strong functional consequences.

Nature Communications · Dec 3, 2024Peer-reviewedEvolutionary Biology