The paper measures translation dynamics in mouse neocortex neurogenesis, revealing a critical period of translational control that regulates protein expression and neuronal differentiation.

Nature Structural & Molecular Biology · Dec 1, 2022Peer-reviewedNeuroscience

Promoter repression and 3D-restructuring resolves divergent developmental gene expression in TADs

Alessa R. Ringel, Quentin Szabo, Andrea M. Chiariello, Konrad Chudzik, Robert Schöpflin, Patricia Rothe, Alexandra L. Mattei, Tobias Zehnder, Dermot Harnett, Verena Laupert, Simona Bianco, Sara Hetzel, Mai H.Q. Phan, Magdalena Schindler, Daniel M. Ibrahim, , Michael I. Robson

The paper shows that divergent gene expression within TADs is resolved through TAD restructuring and DNA methylation, not enhancer-promoter compatibility or position.

bioRxiv · Oct 8, 2021 · PreprintGenetics

The architecture of protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit

Matthew L. Kraushar, Ferdinand Krupp, Dermot Harnett, Paul Turko, Mateusz C. Ambrozkiewicz, Thiemo Sprink, Koshi Imami, Manuel Günnigmann, Ulrike Zinnall, Carlos H. Vieira-Vieira, Theres Schaub, Agnieszka Münster‐Wandowski, Jörg Bürger, Ekaterina V. Borisova, Hiroshi Yamamoto, , Christian M. T. Spahn

The paper reveals Ebp1's role in neuronal proteostasis by analyzing ribosomal architecture and proteomic changes in the developing mouse neocortex.

Molecular Cell · Dec 22, 2020Peer-reviewedNeuroscience

Ribo-seQC: comprehensive analysis of cytoplasmic and organellar ribosome profiling data

Lorenzo Calviello, Dominique Sydow, Dermot Harnett, Uwe Ohler

Ribo-seQC is a software package that evaluates ribosome profiling data quality and translational profiles across cytoplasmic and organellar ribosomes, outputting shareable platform-independent HTML reports.

bioRxiv · Apr 8, 2019 · PreprintBioinformatics