Amy P. Wong

A Dual-Locus-Targeting Strategy to Enhance CRISPR/Cas9-mediated CFTR Replacement via Helper-Dependent Adenoviral vector in porcine genome

The paper develops a dual-locus-targeting strategy using CRISPR/Cas9 and helper-dependent adenoviral vectors to improve CFTR gene replacement in porcine cells.

A novel method for detection of Cas9 gRNAs using a fluorophore-labeled DNA oligo

The paper introduces a method for detecting Cas9 gRNAs using fluorophore-labeled DNA oligos to improve gene editing design and targeting efficiency.

Early human fetal lung atlas reveals the temporal dynamics of epithelial cell plasticity.

This study creates a single-cell atlas of human fetal lung development, revealing epithelial cell plasticity and lineage hierarchies through scRNA-seq and spatial transcriptomics.

Single-cell RNA-Sequencing Co-Expression Analysis with CFTR in Lung Tissue

The paper identifies cell types and modifier genes co-expressed with CFTR in lung tissue using single-cell RNA sequencing to inform therapeutic target prioritization.

A new platform for high-throughput therapy testing on iPSC-derived, immature airway from Cystic Fibrosis Patients

This paper describes a fluorescence-based platform for high-throughput Cystic Fibrosis therapy testing using iPSC-derived immature airway epithelium.

Modeling lung cell development using human pluripotent stem cells

This study uses human pluripotent stem cells to model lung development, generating renewable lung epithelial spheroids and identifying new cell lineage relationships.

Impaired Fetal Lung Development can be Rescued by Administration of Extracellular Vesicles Derived from Amniotic Fluid Stem Cells

The paper demonstrates that extracellular vesicles from amniotic fluid stem cells can rescue impaired fetal lung development in experimental models.