Christof Niehrs

Human PARPs modify RNA nucleobases in vitro and in cells

This study shows PARP10 and PARP15 modify RNA nucleobases in vitro and in cells, and TARG1 reverses these modifications.

PARP1 Writes N3-Cytidine ADP-Ribosylation in DNA

This study identifies PARP1's ability to ADP-ribosylate N3-deoxycytidine in ssDNA, leading to deamination products detectable by LC-MS/MS.

Secretion and signaling properties of Wnt11 and Wnt3 are determined by their N-terminus

This study shows that the N-terminus of Wnt proteins determines their secretion and signaling properties, with Wnt11's N-terminus enabling Evi/Wls-independent secretion and Frizzled 6 activation.

Mammalian N1-adenosine PARylation is a reversible DNA modification

The paper demonstrates that mammalian DNA undergoes reversible N1-adenosine PARylation, identified through mass spectrometry and enzymatic analysis.

DNA methylation clocks for clawed frogs reveal evolutionary conservation of epigenetic ageing

The paper develops DNA methylation clocks for clawed frogs, showing evolutionary conservation of epigenetic ageing mechanisms between frogs and mammals.

Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning

The paper improves m6A RNA modification detection using miCLIP2 and m6Aboost, identifying thousands of high-confidence sites in human and mouse cells without requiring Mettl3 depletion.

R-spondins are BMP receptor antagonists in early embryonic development

This study identifies R-spondins as BMP receptor antagonists, showing they inhibit BMP signaling via ZNRF3 and regulate dorsoventral axis formation in Xenopus embryos.