Ole Østergaard

This study compares extracellular vesicles (EVs) and mechanically induced vesicles (MVs) for membrane protein structure determination, finding that MVs provide better structural representation of HER2 in native environments.

Orbitrap Astral Zoom upgrades enable 270 Hz DIA scanning, identifying 7184 protein groups at 500 samples/day and >12,000 proteins from fractionated HEK293 in 2.7 h.

This study benchmarks enrichment and depletion methods for plasma proteomics across different plasma types and evaluates their correlation with clinical assays.

The paper introduces Narrow-window DIA, a fast proteomics method enabling high-throughput and comprehensive proteome analysis with high precision and accuracy.

The paper develops a high-throughput workflow for subcellular proteome and phospho-proteome profiling and applies it to study EGFR signaling and stress responses in cells.