Samie R. Jaffrey

This study shows that FTO regulates morphine reward and tolerance through m6Am methylation of snRNA, without affecting analgesia.

This study shows that the integrated stress response stabilizes m6A-containing mRNAs, leading to increased protein output despite reduced translation.

This study identifies mRNA glycation by methylglyoxal as a metabolic stress sensor, showing impaired translation and beta-cell dysfunction, which is mitigated by metformin.

The paper evaluates Dorado basecalling for nanopore DRS to detect m6A modifications in RNA, finding that stringent filtering improves accuracy and revealing cell-type differences in m6A proportions at conserved sites.

This study reassesses FTO's role as an m6A eraser in AML using direct RNA nanopore sequencing, finding no effect on m6A stoichiometry but increased m6Am levels.

This study shows that tRNA modification mcm5s2U34 is essential for stress granule formation and melanoma metastasis, suggesting it as a therapeutic target.

The paper introduces CROWN-seq to map m6Am modifications and transcription-start sites in human cell lines, revealing high stoichiometry and links to transcription initiation.

The paper introduces BASH MaP and DAGGER to identify alternative tertiary structures of RNA, revealing conformational dynamics in vitro and in cells.

This study develops a method to produce circular mRNA in cells, enhancing protein expression duration and enabling its use in virus-like particle delivery systems.

The paper shows that m6A methylation drives length-dependent mRNA enrichment in stress granules by enabling YTHDF-mediated mRNA interactions.

This study identifies Cbln1 as a novel axon growth and guidance cue in multiple CNS regions, revealing its role in early development and its interaction with Nrxn2.

The paper investigates the source of METTL3-independent m6A in mRNA, finding that most m6A is due to METTL3 and that apparent knockouts often have altered but functional METTL3 isoforms.

The paper introduces a method to specifically detect m6A in mRNA from total RNA samples, addressing biases from mRNA purification protocols.

This study evaluates the reproducibility of m6A peak detection and changes using MeRIP-seq and finds limited consistency across studies.

The paper identifies N6-methyladenosine in poly(A) tails of VSG transcripts in Trypanosoma brucei, showing it regulates mRNA stability through a cis-acting motif.

The paper shows that m1A is only present in the mitochondrial MT-ND5 transcript, and previous findings of m1A in 5 UTRs were due to antibody cross-reactivity with m7G caps.

The paper introduces CapQuant, a mass spectrometry-based method to analyze RNA caps, revealing new cap structures and variations in humans and mice.

The paper identifies PCIF1 as the m6Am methyltransferase and develops a method to accurately map m6Am sites, revealing its role in mRNA stability.

This study shows FTO demethylates m2-snRNA to regulate splicing by controlling reversible methylation of snRNAs.