Sarah R. Ocañas

The paper shows that cell identity and regulatory logic are preserved during brain aging, but cell-type-specific changes occur in DNA modifications and gene expression.

The paper identifies conserved sex-biased microglial responses to aging and amyloid pathology in mice and humans using single-cell transcriptomics and proteomics.

This study shows that tamoxifen temporarily disrupts estrous cycles but does not alter long-term ovarian aging patterns or transcriptional outcomes.

This paper describes a protocol for isolating five specific brain cell types from a single mouse brain using magnetic-activated cell sorting.

This study identifies age-related changes in chromatin accessibility in female microglia, showing more gains than losses in accessibility, enriched in immune-related genes and intergenic regions.

The paper identifies differential DNA modifications across CNS cell types and their relationship with gene expression using a novel mouse model and epigenomic sequencing.

The paper compares tamoxifen-mediated cre induction efficiency and specificity in young and old mice, finding no significant difference in microglial labeling or translatomic changes.

The paper identifies sex differences in microglial transcriptomic and translatomic profiles in aging mouse hippocampus, highlighting female-biased senescence and disease-associated signatures linked to Alzheimer's disease.

The paper identifies microglia as the primary source of MHC-I in mice and humans, showing age-related and Alzheimer's-associated increases in MHC-I expression and related receptors.

The paper investigates how chromosomal content and gonadal sex influence gene expression and DNA modification patterns in mouse hippocampi, revealing sex chromosome-specific regulatory effects.

The paper compares microglia isolation techniques and shows that transcription/translation inhibitors reduce ex vivo activation artifacts during cell preparation.

The paper introduces a NuTRAP method to isolate RNA and DNA from specific CNS cell types, revealing cell type-specific epigenetic and transcriptomic differences in microglia and astrocytes.

This study finds that male mice show age-related decline in substantia nigra dopamine neuron firing, while females remain resilient, with increased expression of Parkinson's-related genes in males.