Kevin D. Pham

A new epigenetic framework for DNA methylation, hydroxymethylation, and gene expression during brain aging.

The paper shows that cell identity and regulatory logic are preserved during brain aging, but cell-type-specific changes occur in DNA modifications and gene expression.

Functional and molecular profiles of the neuroretina and retinal pigmented epithelium-choroid in response to aging and Nrf2 deletion

The paper examines how aging and Nrf2 deletion affect visual function and gene expression in the retina and RPE-choroid, finding exacerbated age-related declines in Nrf2-deficient mice.

Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting

This paper describes a protocol for isolating five specific brain cell types from a single mouse brain using magnetic-activated cell sorting.

Parallel gene expression changes in ventral midbrain dopamine and GABA neurons during normal aging

The paper examines gene expression changes in dopamine and GABA neurons during aging in mice, identifying shared and distinct molecular signatures linked to neurodegenerative disease susceptibility.

VTA dopamine neurons are hyperexcitable in 3xTg-AD mice due to casein kinase 2-dependent SK channel dysfunction

This study identifies hyperexcitability in VTA dopamine neurons of 3xTg-AD mice due to CK2-dependent SK channel dysfunction, linking it to reward-based learning deficits.

Late-life short-term dietary restriction ameliorates intestinal stem cell function and impacts intestinal stem cell DNA methylation

This study shows that late-life short-term dietary restriction improves intestinal stem cell function and alters DNA methylation in aged mice.

Changes in microglia chromatin accessibility in aged female mice.

This study identifies age-related changes in chromatin accessibility in female microglia, showing more gains than losses in accessibility, enriched in immune-related genes and intergenic regions.

Differential usage of DNA modifications in neurons, astrocytes, and microglia

The paper identifies differential DNA modifications across CNS cell types and their relationship with gene expression using a novel mouse model and epigenomic sequencing.

Consistent specificity and efficiency of tamoxifen-mediated cre induction across ages

The paper compares tamoxifen-mediated cre induction efficiency and specificity in young and old mice, finding no significant difference in microglial labeling or translatomic changes.

Microglial senescence contributes to female-biased neuroinflammation in the aging mouse hippocampus: implications for Alzheimer's disease

The paper identifies sex differences in microglial transcriptomic and translatomic profiles in aging mouse hippocampus, highlighting female-biased senescence and disease-associated signatures linked to Alzheimer's disease.

Microglial MHC-I induction with aging and Alzheimer's is conserved in mouse models and humans

The paper identifies microglia as the primary source of MHC-I in mice and humans, showing age-related and Alzheimer's-associated increases in MHC-I expression and related receptors.

Differential regulation of mouse hippocampal gene expression sex differences by chromosomal content and gonadal sex

The paper investigates how chromosomal content and gonadal sex influence gene expression and DNA modification patterns in mouse hippocampi, revealing sex chromosome-specific regulatory effects.

Minimizing the ex vivo confounds of cell-isolation techniques on transcriptomic profiles of purified microglia

The paper compares microglia isolation techniques and shows that transcription/translation inhibitors reduce ex vivo activation artifacts during cell preparation.