Walker Hoolehan

A new epigenetic framework for DNA methylation, hydroxymethylation, and gene expression during brain aging.

The paper shows that cell identity and regulatory logic are preserved during brain aging, but cell-type-specific changes occur in DNA modifications and gene expression.

Methylation Array Signals are Predictive of Chronological Age Without Bisulfite Conversion

The paper shows that non-methylation factors cause age-predictive pseudomethylation signals in methylation arrays, affecting epigenetic clocks.

Changes in microglia chromatin accessibility in aged female mice.

This study identifies age-related changes in chromatin accessibility in female microglia, showing more gains than losses in accessibility, enriched in immune-related genes and intergenic regions.

Differential usage of DNA modifications in neurons, astrocytes, and microglia

The paper identifies differential DNA modifications across CNS cell types and their relationship with gene expression using a novel mouse model and epigenomic sequencing.

High Throughput Characterization of V(D)J Recombination Signal Sequences Redefines the Consensus Sequence

The paper develops a high-throughput assay to analyze V(D)J recombination signal sequences (RSSs) and finds RAG1/2 activity is influenced by DNA structural features rather than strict sequence conservation.