Willard M. Freeman

The paper shows that cell identity and regulatory logic are preserved during brain aging, but cell-type-specific changes occur in DNA modifications and gene expression.

The paper identifies conserved sex-biased microglial responses to aging and amyloid pathology in mice and humans using single-cell transcriptomics and proteomics.

The paper examines how aging and Nrf2 deletion affect visual function and gene expression in the retina and RPE-choroid, finding exacerbated age-related declines in Nrf2-deficient mice.

This study shows that hepatocyte MLKL promotes obesity-driven MASLD-HCC through mitochondrial dysfunction and MFN2 suppression, independent of necroptosis.

This paper describes a protocol for isolating five specific brain cell types from a single mouse brain using magnetic-activated cell sorting.

The paper shows that non-methylation factors cause age-predictive pseudomethylation signals in methylation arrays, affecting epigenetic clocks.

The paper examines gene expression changes in dopamine and GABA neurons during aging in mice, identifying shared and distinct molecular signatures linked to neurodegenerative disease susceptibility.

This study identifies hyperexcitability in VTA dopamine neurons of 3xTg-AD mice due to CK2-dependent SK channel dysfunction, linking it to reward-based learning deficits.

This study shows that late-life short-term dietary restriction improves intestinal stem cell function and alters DNA methylation in aged mice.

This study identifies age-related changes in chromatin accessibility in female microglia, showing more gains than losses in accessibility, enriched in immune-related genes and intergenic regions.

The paper identifies differential DNA modifications across CNS cell types and their relationship with gene expression using a novel mouse model and epigenomic sequencing.

The paper compares tamoxifen-mediated cre induction efficiency and specificity in young and old mice, finding no significant difference in microglial labeling or translatomic changes.

The paper identifies sex differences in microglial transcriptomic and translatomic profiles in aging mouse hippocampus, highlighting female-biased senescence and disease-associated signatures linked to Alzheimer's disease.

The paper identifies microglia as the primary source of MHC-I in mice and humans, showing age-related and Alzheimer's-associated increases in MHC-I expression and related receptors.

This study shows that deleting melanocortin-4 receptors on hypothalamic astrocytes increases body weight, feeding, and central inflammation, independent of neuronal MC4R effects.

The paper investigates how chromosomal content and gonadal sex influence gene expression and DNA modification patterns in mouse hippocampi, revealing sex chromosome-specific regulatory effects.

This study shows that litter expansion in mice leads to long-term metabolic changes that are sex-dependent, with males showing more pronounced effects than females.

The paper compares microglia isolation techniques and shows that transcription/translation inhibitors reduce ex vivo activation artifacts during cell preparation.

The paper introduces a NuTRAP method to isolate RNA and DNA from specific CNS cell types, revealing cell type-specific epigenetic and transcriptomic differences in microglia and astrocytes.

This study finds that male mice show age-related decline in substantia nigra dopamine neuron firing, while females remain resilient, with increased expression of Parkinson's-related genes in males.

This study shows that cigarette smoke activates NOTCH3, promoting goblet cell differentiation in human airway epithelial cells, contributing to COPD-related goblet cell metaplasia.

The paper shows that early life DNA methylation patterns predict age-related transcriptome changes in mice, supporting the developmental origins of disease hypothesis.

This study shows that caloric restriction reduces age-related DNA methylation changes in the hippocampus of mice, including both CG and non-CG sites, and introduces CR-specific methylation patterns linked to gene expression changes.

This study identifies sexually divergent DNA methylation changes in mouse and human hippocampus with aging, revealing sex as a key factor in epigenomic aging.