Bacterial Genetics and Biotechnology

The paper identifies PA3962 as a periplasmic metallochaperone (PmcY) that couples Zn2+ transport to sensing in Pseudomonas aeruginosa, regulating OprD expression and imipenem sensitivity.

The paper shows that second messengers in E. coli affect gene expression through transcriptional competition, reshaping proteome allocation beyond individual regulatory effects.

The paper analyzes Lgt homologues across bacterial pathogens to determine functional diversity and catalytic mechanisms, identifying a conserved motif and substrate specificity differences.

The paper shows that a pseudogenization in the cntQ gene alters metal uptake specificity in Yersinia species, shifting from iron to zinc.

This paper uses FLIM-FRET to visualize FtsW protein interactions in live Staphylococcus aureus, revealing self-interactions and interactions with PBP1 and DivIB, and showing how antibiotics affect these interactions.

This study identifies SuhB as a global post-transcriptional regulator in Sphingopyxis granuli TFA, showing its role in stress resistance and metabolic balance through proteomic and genetic analyses.

This study identifies two categories of extrachromosomal replicons in bacteria and shows that large replicons replicate in sync with the chromosome and use its replication machinery for stable inheritance.

This study reveals the structural mechanism by which Bacteroides thetaiotaomicron steals iron-bound siderophores from pathogens using the XusAB complex.

This paper shows that cAMP inputs into a bistable regulatory network drive subpopulations of Pseudomonas aeruginosa cells to prime for T3SS expression.

This paper investigates the role of SanA in maintaining envelope integrity in E. coli by linking its absence to increased lipid II availability and imbalanced septal peptidoglycan synthesis.

This study uses live-cell single-molecule tracking to analyze how NAPs like Dps and H-NS affect HU-DNA interactions across different growth phases in E. coli.

The paper identifies water-contact residues in GerAB that are critical for germinosome stability in Bacillus subtilis spores, based on MD simulations and functional assays.

Ligify 2.0 predicts small molecule interactions with transcription factors and provides a web interface for visualization and biosensor design.

This study determines the structure and mechanism of UbK1, a bacterial kinase in P. gingivalis, identifies PorX as a noncanonical substrate, and shows UbK1's role extends beyond canonical secretion pathways.

This study investigates how S. aureus traverses nanoscale channels in bone, finding that fluid pressure, not cell division, enables bacterial entry into narrow spaces.

This paper develops a tetracycline-inducible gene expression system for Bacillus subtilis with high dynamic range and minimal leakiness across growth conditions.

This study shows that LytF promotes pilus extrusion in Streptococcus sanguinis, enhancing DNA uptake during natural transformation, contrary to previous speculation that it functions as a fratricin.

The paper develops a mathematical model to explain memory in Bacillus subtilis spore germination, showing that GR states and SpoVA channel dynamics are crucial for memory.

The paper measures PMF dynamics in E. coli under hyperosmotic stress using the flagellar motor as a voltmeter, finding rapid and reversible PMF loss.

This study identifies a two-component system signaling hub that controls membrane remodeling in Enterococcus faecalis in response to daptomycin, linking lipid changes to antibiotic resistance.

This study investigates the TAM protein complex in Pseudomonas aeruginosa, showing TamA is a potential drug target due to its role in virulence and OM protein assembly.

This paper identifies ectoine catabolism pathways in diverse bacterial and archaeal species, showing their evolutionary distribution and environmental relevance.

This study identifies CuiT as a key copper importer in Salmonella enterica, showing its role in copper homeostasis, gene regulation, and stress adaptation.

The paper shows that the ParMR system in Anabaena sp. has evolved into a cytoskeletal system, CorMR, that controls cell shape instead of DNA segregation.

This study uses ChIP-seq to analyze transcription factor binding sites in Pseudomonas aeruginosa, revealing regulatory networks, master virulence regulators, and a database for TF-binding sites.

This study identifies two distinct mechanisms by which PomX and PomY activate PomZ ATPase in Myxococcus xanthus, revealing a non-canonical AAP mechanism for PomY and a role analogous to MinE for PomX.

This study shows that SmRNase III in Sinorhizobium meliloti affects gene expression under microaerobic conditions, impacting nitrogen fixation and sRNA-mediated regulation.

This study identifies growth medium-specific important genes in Escherichia coli using transposon mutagenesis and sequencing, revealing a core set of essential genes and medium-specific gene modules.

The paper shows host-associated bacteria lose flagellar genes more than free-living ones, with little compensation from horizontal gene transfer.

This paper identifies a small molecule regulated by a TetR family regulator that controls the GA3 T6SS and immunity genes in Bacteroides fragilis, enabling coordinated antagonism and defense.