Bacterial Genetics and Biotechnology

This study identifies HpaAB as an active chromosome segregation system in Haloferax volcanii, essential for equipositioning replicons and maintaining genomic stability.

This study shows that pressure-induced membrane tension opens the GerA ion channel in Bacillus subtilis spores, triggering germination through a stretch-to-open mechanism.

The paper investigates the phylogenetic correlation between HIP1 and Type IV Secretion System proteins in cyanobacteria, suggesting HIP1 facilitates horizontal gene transfer.

The paper identifies lysinoalanine crosslinking in the extracellular flagellar hook of Synergistota, expanding the known range of bacteria with this modification beyond spirochetes.

This study shows H-NS in E. coli undergoes a conformational switch at 37°C, enabling nucleoid reorganization that enhances growth, resolving the silencing paradox.

The paper shows that single-cell growth rates increase with flagellar gene activity, contradicting bulk measurements, and attributes this to inherited growth factors.

This paper identifies mechanisms of amino acid toxicity in S. aureus and shows glycine can enhance antibiotic efficacy when combined with D-cycloserine.

The paper investigates how alternative respiratory electron transport pathways affect aminoglycoside susceptibility in bacteria.

This study identifies GndA, a microprotein in Escherichia coli, which promotes cell growth during heat shock independently of its overlapping 6PGD protein.

This paper shows BR-bodies in Brucella regulate RNA stability and virulence by linking phase separation to stress resistance and intracellular fitness.

This study identifies minJ and divIVA as essential for chromosome segregation in Bacillus subtilis when SMC function is impaired, highlighting the interdependence between chromosome segregation and cell division.

This study identifies IscR-mediated morphological changes in A. baumannii that reduce stress molecule uptake, enhancing virulence and survival under oxidative stress and antibiotic treatment.

This paper identifies DD-CPases as the source of tetrapeptides for LDTs in C. difficile and shows that LDTs are essential due to high DD-CPase activity, not for divisome/elongasome function.

This study investigates MOMP assembly in Corynebacterium glutamicum, showing that PorH and ProtX are inserted at polar growth zones and linked to the cell wall.

The paper identifies SamI as a cytosolic SPFH protein that couples membrane stress to differentiation in Bacillus subtilis by stabilizing a membrane complex involved in stress signaling and biofilm formation.

This paper shows that horizontally acquired mgtC genes can have different physiological roles depending on genomic and biochemical contexts in Salmonella enterica and other bacteria.

This paper shows that phosphorylation by PknB at T101 promotes WalR dimer formation and strengthens DNA binding, revealing a direct functional link between PknB and WalR.

This study uses CRISPRi to screen B. subtilis genes across 150 stress conditions, revealing new roles for conserved firmicute genes in protein secretion, cell wall homeostasis, and bacterial competition.

The paper identifies FtsZ paralogs in Hyphomicrobiales, showing they may fine-tune cell division, with FtsZ1 inhibiting division when overexpressed in Agrobacterium tumefaciens.

The paper develops a synthetic system to screen chimeric DcuS/EnvZ histidine kinases and identifies key residues affecting ligand responsiveness and selectivity.

This study compares acid stress resistance between Salmonella Typhimurium and Salmonella Typhi, finding that Typhi has impaired growth at acidic pH due to gene loss and pseudogenization.

This study identifies CtiC, a fertility inhibitor encoded by SGI1 that blocks conjugative transfer of IncA/C plasmids by interfering with relaxosome assembly.

This study identifies how SpNanR activates sialic acid metabolism in S. pneumoniae by binding N-acetylmannosamine-6-phosphate, stabilizing its tetramer and enhancing transcription of the nan and siaA operons.

This paper identifies SepIVA as a divisome activator in M. smegmatis, showing it is inhibited during DNA damage by disrupting its interaction with FtsQ.

This study shows that chloramphenicol induces antibiotic resistance in Bacillus subtilis by upregulating resistance genes through translational regulation, revealing new resistance mechanisms against glycopeptides.

This study examines mRNA-protein level correlations in bacterial pathogens under stress, finding reduced correlations and highlighting the role of post-translational modifications in stress adaptation.

This study identifies a lipid cue that drives the subcellular localization of a bacterial transmembrane protein, ShfA, by binding to undecaprenyl phosphate.

This study shows that acetylation of CRP K52 activates AR3, leading to changes in gene expression that affect sugar utilization and virulence in V. cholerae.

This study defines the S. aureus CymR-dependent and -independent sulfur starvation responses using chemically defined growth conditions and identifies key sulfur sources and their regulatory mechanisms.

The paper identifies a stress-induced membrane insertion mechanism that activates the BepA metalloprotease by displacing its autoinhibitory plug, linking membrane association to protease function.