Lesley Hoyles

Genome-resolved surveillance of African Klebsiella oxytoca species complex genomes reveals resistome-mobilome and biosynthetic gene cluster diversity

The paper analyzes African Klebsiella oxytoca species complex genomes to characterize genomic diversity, resistance, and biosynthetic gene clusters across multiple species.

Hippurate hydrolases encoded by Klebsiella pneumoniae and Klebsiella oxytoca are functional

The paper confirms that Klebsiella pneumoniae and Klebsiella oxytoca can hydrolyse hippurate, identifying functional hippurate hydrolases in these species.

A capsule polysaccharide synthesis locus database for the Klebsiella oxytoca Species Complex

A curated Kaptive-compatible database of 88 capsule (K) loci types 97.6% of dereplicated Klebsiella oxytoca species complex genomes, versus 50.3% typed with the existing K. pneumoniae database.

Comparative analyses of Gram-negative bacteria isolated from cancer patients with bacteraemia at the Uganda Cancer Institute

This study shows that transporting bacterial DNA at ambient temperature from Uganda to the UK allows high-quality whole-genome sequencing, revealing AMR patterns and misidentification issues in Gram-negative bacteria from cancer patients.

The Omp85 family protein, TamA, exhibits characteristics 1 of a suitable drug target against Pseudomonas aeruginosa

This study investigates the TAM protein complex in Pseudomonas aeruginosa, showing TamA is a potential drug target due to its role in virulence and OM protein assembly.

Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control

This study identifies trimethylamine (TMA) as a microbial compound that inhibits IRAK4, reducing inflammation and improving metabolic outcomes in high-fat diet conditions.

Phenotype-genotype discordance in antimicrobial resistance profiles of Gram-negative uropathogens recovered from catheter-associated urinary tract infections in Egypt

This study compares phenotypic and genomic AMR profiles of Gram-negative uropathogens from CAUTIs in Egypt, finding significant phenotype-genotype discordance and reporting new AMR variants.

Growth of Gram-negative uropathogens in human urine under hypoxic conditions produces clinically relevant metabolomic profiles

This study shows that growing Gram-negative uropathogens in human urine under hypoxic conditions produces metabolomic profiles more relevant to clinical disease than aerobic conditions or artificial urine.

Weberviruses are gut-associated phages that infect Klebsiella spp.

The paper identifies and characterizes seven new lytic phages infecting Klebsiella, confirms their classification as Webervirus, and identifies a new genus, while highlighting their potential as therapeutic agents against multidrug-resistant strains.

Menadione reduces the expression of virulence- and colonisation-associated genes in Helicobacter pylori

This study shows that menadione reduces virulence and colonization gene expression in H. pylori and inhibits motility, suggesting potential as a virulence-attenuating agent.

Cerebrovascular damage caused by the gut microbe-derived uraemic toxin p-cresol sulfate isprevented by blockade of the epidermal growth factor receptor

This study shows p-cresol sulfate impairs the blood-brain barrier via EGFR activation and demonstrates EGFR blockade prevents this damage in vitro.

The plasmid-borne hipBA operon of Klebsiella michiganensis encodes a potent plasmid stabilization system

This paper identifies and characterizes a plasmid-encoded HipBA toxin-antitoxin system in Klebsiella michiganensis that contributes to plasmid stability and antibiotic persistence.

A host-gut microbial co-metabolite of aromatic amino acids, p-cresol glucuronide, promotes blood-brain barrier integrity in vivo

This study shows that p-cresol glucuronide, a host-gut microbial co-metabolite, reduces blood-brain barrier permeability and modulates brain transcriptome expression in mice.

Regulation of blood-brain barrier integrity by microbiome-associated methylamines and cognition by trimethylamine N-oxide

This study shows that TMAO enhances BBB integrity and protects against inflammation, while TMA impairs BBB function and cognitive resilience in mice.

Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synaptic plasticity- and neurotransmission-related proteins in young recipients

This study shows that FMT from aged mice impairs spatial learning in young recipients by altering hippocampal proteins related to synaptic plasticity and neurotransmission.

Rapid profiling of the preterm infant gut microbiota using nanopore sequencing aids pathogen diagnostics

This study uses nanopore sequencing to rapidly profile preterm infant gut microbiota, enabling pathogen detection and antimicrobial resistance profiling within one hour.

Microbiome Determinants and Physiological Effects of the Benzoate-Hippurate Microbial-Host Co-Metabolic Pathway

This study identifies microbiome determinants of hippurate, a metabolite linked to metabolic health, and demonstrates its protective effects in mice through benzoate-hippurate pathway analysis.

A network-based data-mining approach to investigate indole-related microbiota-host co-metabolism

The paper identifies prokaryotes involved in indole metabolism using KEGG and BLAST integration, and provides resources for further validation.

Microbiome-host systems interactions: Protective effects of propionate upon the blood-brain barrier

The paper investigates how propionate, a gut microbial metabolite, interacts with the blood-brain barrier through CD14-dependent and NRF2 signaling pathways, suggesting a new mechanism in the gut-brain axis.