Bacterial Genetics and Biotechnology

This study shows that BctCBA in B. pertussis binds citrate-chelated Zn2+ and Ni2+, revealing a novel metal acquisition mechanism.

This study identifies PepA as a regulator of nhaB gene expression in Vibrio cholerae, revealing its role in Na+ homeostasis through suppressor mutations in a nhaA nqr mutant.

The paper quantifies developmental phenotypes of a Myxococcus xanthus mutant lacking TodK, revealing its role in modulating biofilm development through regulation of MrpC.

The paper introduces CIM, a method to predict CFM-Rec interactions from genomic data, revealing topological patterns in microbial communities.

The paper uses a statistical mechanics model to show that stator pool size determines the load range over which the bacterial flagellar motor is mechanosensitive.

This paper identifies MicC as a multifunctional regulator of ExPEC fitness and virulence across host niches by modulating multiple pathways beyond its known target OmpC.

The paper identifies genes required for smooth lipopolysaccharide (S-LPS) production in E. coli K-12 through a genome-wide screen, revealing 27 mutants with defects in S-LPS production and highlighting the need for validation.

This study visualizes bacterial nanotube formation in Bacillus subtilis, revealing a novel membranous organelle called the tubeosome that facilitates intercellular communication.

This study examines how different cell wall antibiotics affect peptidoglycan synthesis in Bacillus subtilis, revealing distinct morphological outcomes and mechanisms of cell lysis.

This study investigates the role of PopZ in Zymomonas mobilis, finding it dispensable for growth but necessary for polar localization of ParB.

The paper develops a high-throughput method to profile bacterial respiration using the Resipher, showing it can detect functional responses to nutrients and antibiotics.

This paper identifies a geometry sensing oscillator in archaea that spatially regulates cell division, showing convergent evolution with bacterial systems.

This paper identifies the WalRK two-component system's role in maintaining cell envelope integrity by compensating for reduced capsule and wall teichoic acid attachment in Streptococcus pneumoniae.

The paper shows bacteriostatic antibiotics can drive bacterial death by altering competitive interactions between strains, not directly killing bacteria.

This study identifies glycerol metabolism as critical for antibiotic tolerance in stationary-phase Escherichia coli by maintaining energy homeostasis and proteostasis.

The paper shows how promoter architecture in Pseudomonas aeruginosa determines whether phosphorylation of the ParR response regulator activates, represses, or reverses gene expression.

This study investigates chromosome organization in obligate mammalian symbionts, revealing conserved architectures linked to their lifestyle and growth conditions.

This study shows that the lytic transglycosylase SleB in Zymomonas mobilis is crucial for cell fitness and stress tolerance through its role in peptidoglycan remodeling.

This study identifies ROS detoxification as critical for antibiotic survival in Mycobacterium abscessus, showing that antibiotics induce ROS which tolerant cells must neutralize.

The paper uses machine learning to compare transcriptional regulatory networks of three Pseudomonas strains, revealing shared and unique regulatory mechanisms across species.

The paper investigates methyltransferases (MTs) in CPR genomes, finding they are primarily associated with restriction-modification systems for DNA defense and nucleotide scavenging rather than antimicrobial resistance.

The paper traces the origin of hopanoids to marine alphaproteobacteria and identifies new gene families involved in their trafficking.

The paper investigates the roles of core promoter elements -35 and -10 in Escherichia coli, showing -10 is essential for promoter evolution while -35 influences gene regulation through interactions with transcription factors.

The paper identifies how VncS cross-phosphorylates RR06 to activate CbpA, a key adhesin in Streptococcus pneumoniae, resolving a mechanistic controversy.

This study identifies ten cell division proteins in Haloferax volcanii using CLIP-MS, with CdpC functioning as a membrane anchor for FtsZ1 and containing zinc fingers critical for its role.

This study shows how promoter architecture in Pseudomonas aeruginosa determines different transcriptional outputs from the same ParR phosphorylation signal.

This paper identifies HigX as a membrane-associated transcription factor in Caulobacter crescentus that is induced by the SOS response but negatively affects cell viability, revealing its role in cell envelope regulation and the trade-off between DNA repair and cell envelope stability.

This paper identifies a one-component regulatory system in marine Bacteroidota that controls laminarin utilization, showing it activates gene clusters and operates without phosphorylation.

The paper identifies a missing component of the ComB T4SS in H. pylori essential for DNA uptake, showing it is a hexameric ATPase interacting with ComB4.

This paper identifies CenIR as an essential BlaIR-family regulatory system in C. difficile that lacks a β-lactam-binding domain and is required for viability, with its regulon containing genes involved in peptidoglycan hydrolase and cell envelope maintenance.