Reproductive biology and impacts on aquatic species

A scalable platform for functional interrogation of peptide hormones in fish

The paper introduces a high-throughput platform for functional studies of peptide hormones in fish, demonstrating its ability to rescue growth and fertility defects through genetic manipulation.

Cold Storage and Cryopreservation Methods for Spermatozoa of the Sea Urchin, Lytechinus pictus .

The paper develops methods to cryopreserve sperm from two sea urchin species, maintaining fertilization capacity and producing viable larvae after multiple freeze-thaw cycles.

Fast, safe and high-throughput Real-Time PCR protocol for molecular sex identification in Salmo salar, applicable to historic scale collections

The paper develops a Real-Time PCR protocol for sex identification in Atlantic salmon, demonstrating accuracy and applicability to degraded DNA samples.

Defining the cellular complexity of the zebrafish bipotential gonad

The paper identifies the arrival and interactions of vascular, lymphatic, pericyte, and macrophage cells in the zebrafish bipotential gonad during early development.

Downstream effects of dre-miR-210-5p in zebrafish primary ovarian cell culture

This study investigates the effects of dre-miR-210-5p in zebrafish ovarian cells using an in vitro model, identifying thousands of differentially expressed genes and their involvement in reproductive and immune pathways.

The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

This study identifies Adad1 as essential for germ cell maintenance and meiosis in zebrafish, contrasting with its role in mice.

Membrane molecule bouncer enables follicle fertilization in a viviparous teleost: Poecilia reticulata (guppy)

The paper identifies bouncer as a key protein enabling immature oocyte fertilization in guppies, a unique reproductive strategy not observed in other fish species.

Genomic disruption of type II vitellogenin leads to vitellin membrane deficiencies andsignificant mortalities at early stages of embryonic development in zebrafish (Danio rerio)

This study uses CRISPR/Cas9 to disrupt vtg2 in zebrafish, revealing its essential role in vitellin membrane formation and early embryonic survival.

Distinct features in fish Bouncer proteins determine sperm-egg compatibility

The paper identifies specific amino acid residues and N-glycosylation patterns in Bouncer proteins that mediate species-specific sperm-egg compatibility between medaka and zebrafish.

Photoperiod regulates gonadotrope cell division in medaka via melatonin, Tsh and folliculostellate cells

This study shows that in medaka, photoperiod regulates gonadotrope cell division and hormone production through melatonin, TSH, and folliculostellate cells.

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity of parthenogenetic Artemia

This study characterizes ovarian development stages and identifies VgR gene silencing as a factor reducing fecundity in parthenogenetic Artemia.

Validation of a male-specific DNA marker confirms XX/XY-type sex determination in African catfish (Clarias gariepinus)

The paper validates a male-specific DNA marker (CgaY1) in African catfish, confirming an XX/XY sex determination system and demonstrating its accuracy in predicting sex.

Production of sterile Atlantic salmon by germ cell ablation with antisense oligonucleotides

This paper demonstrates the sterilization of Atlantic salmon embryos using antisense oligonucleotides targeting the dead end gene, resulting in germ cell-depleted fish with normal morphology but no gametes.

Sexually dimorphic regulation of gonadotrope cell hyperplasia in medaka pituitary via mitosis and transdifferentiation

This study investigates how sex steroids regulate gonadotrope cell number and function in medaka, revealing sexually dimorphic mechanisms involving mitosis and transdifferentiation.

Phenotypical Rescue of Bmp15 Deficiency by Mutation of Inhibin α (inha) Provides Novel Clues to How Bmp15 Controls Zebrafish Folliculogenesis

This study shows that bmp15 deficiency in zebrafish causes follicle arrest and sex reversal, which can be rescued by inhibin mutation, revealing interactions between bmp15 and the activin-inhibin system in folliculogenesis.

Loss of growth differentiation factor 9 causes an arrest of early folliculogenesis in zebrafish - a novel insight into its action mechanism

This study shows that loss of Gdf9 in zebrafish causes early follicle arrest and sex reversal, which can be rescued by inhibin mutation, suggesting Gdf9 acts via the activin-inhibin system.

Tetraspanin Cd9b plays a role in fertility in zebrafish

This study shows that cd9b mutation in zebrafish reduces egg numbers and fertilization, with different effects depending on the gender of the mutant.

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

The paper identifies sex-specific miRNAs in zebrafish gonads that respond to heat treatment, suggesting their potential as epigenetic markers for environmental exposure.

Assessment of fish spermatogenesis through high-quality immunofluorescence against fish testicular samples with an antibody set

The paper develops antibodies and an immunofluorescence method to accurately identify spermatogenic cell types in fish testis samples across multiple species.

A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish

This study identifies distinct biological processes in zebrafish gonad differentiation at 25 and 30 dpf, revealing metabolic and immune-related pathways in ovary and testis development respectively.

Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary

The paper uses single-cell RNA sequencing to analyze zebrafish ovarian cells, identifying novel cell subpopulations and gene functions involved in ovarian development.

Development of the first antibody targeting vitellogenin using the Palinurus elephas mRNA molecular pathway

The paper develops an antibody targeting vitellogenin in the red spiny lobster Palinurus elephas using its mRNA molecular pathway.

Clonal gametogenesis is triggered by intrinsic stimuli in the hybrids germ cells but is dependent on sex differentiation

The paper investigates the triggers of clonal gametogenesis in hybrids, finding that it is initiated by intrinsic signals in hybrid germ cells but requires a female gonadal environment for execution.

Recurrent Duplication and Diversification of Acrosomal Fertilization Proteins in Abalone

The paper shows how duplication and sequence diversification of acrosomal proteins in abalone contribute to molecular diversity in fertilization.

Molecular mechanisms involved in Atlantic halibut (Hippoglossus hippoglossus) egg quality: impairments at transcription and protein folding levels induce inefficient protein and energy homeostasis during early development

This study identifies differences in proteomic profiles between good and poor quality Atlantic halibut eggs, showing impaired transcription and protein folding in poor quality eggs affecting early development.

Sex-Specific Genes Identified in Sea Urchin Gonads are Expressed Prior to Metamorphosis

The paper identifies sex-specific genes in sea urchin gonads that are expressed before metamorphosis, revealing early sex determination and unique gonad-specific transcripts.

The sperm protein Spaca6 is essential for fertilization in zebrafish

This study shows that zebrafish Spaca6 is essential for fertilization by regulating Dcst2 levels and enabling sperm-egg membrane binding.

The germline-specific region of the sea lamprey genome plays a key role in spermatogenesis

The paper identifies genes in the germline-specific region of the sea lamprey genome and shows their enrichment in testis expression and potential role in sex determination.

Neurodevelopment vs. the immune system: complementary contributions of maternally-inherited gene transcripts and proteins to successful egg development in fish

This study investigates the distinct roles of maternally-inherited mRNAs and proteins in fish egg development, showing they contribute to neurodevelopment and immune protection respectively.

Non-invasive sex genotyping of paiche Arapaima gigas by qPCR: An applied bioinformatic approach to identify sex differences

This paper develops a non-invasive qPCR method for sexing paiche fish using DNA markers identified through a novel bioinformatic approach.