Discover, rate, and discuss biology preprints.

The paper introduces a computational method to calculate minimum energy pathways in transport proteins, providing mechanistic insights into conformational changes in three membrane transporter superfamilies.

The paper derives a solubility product model for biomolecular phase boundaries, showing it accounts for variable stoichiometry and soluble oligomers, revealing curvature and re-entrant behavior in phase boundaries.

The paper investigates whether β2 and β3a BK channel regulatory subunits can coassemble in the same channel complex and finds they do so randomly.

This paper introduces a lensless fiber-optic quantum-sensing endomicroscopy to map temperature at cellular resolution in the mouse deep brain, revealing heterogeneous temperature changes during anesthesia.

The paper compares three computational methods for modeling collaborative DNA methylation dynamics and shows how methylation patterns depend on CpG cluster architecture and enzymatic parameters.

This study investigates spheroid fusion dynamics by measuring cell-cell tension and motility, revealing how tissue fluidity and visco-elastic properties influence fusion outcomes.

This study uses molecular dynamics simulations to analyze water dynamics at the HA-DPPC interface, revealing heterogeneous diffusion behavior and its dependence on HA concentration and chain length.

This paper uses molecular dynamics simulations at 310 K to analyze cryo-EM reconstructions of actin filaments, revealing conformational differences from physiological conditions and explaining functional properties.

BeadBuddy corrects chromatic errors in single-molecule imaging data using fluorescent bead images, achieving sub-voxel resolution in DNA FISH and applicable across SMLM modalities.

This study measures cellular traction forces during confined migration through tunable microscale geometries, revealing that softer constrictions cause greater nuclear deformation due to internal contractile forces.

This study uses remission spectroscopy to show bedaquiline inhibits ATP synthase in mycobacteria, redirecting electron flux through cytochrome bd oxidase and increasing O2 consumption, without uncoupling.

The paper identifies a domain-swapped architecture in latent TGF-β, crucial for its activation through dynamic allostery.

The paper uses crystallographic ensembles to quantify conformational entropy changes in SARS-CoV-2 Macrodomain upon ligand binding, linking structural heterogeneity to binding thermodynamics.

The paper shows CTCF controls chromatin boundary permeability through a dynamic conformational ensemble, not static occupancy, influencing cohesin capture probabilities.

The paper identifies and characterizes far-red-sensitive opsins in mantis shrimp eyes, revealing their spectral tuning mechanisms and photoreversibility.

This study characterizes SaGpx, a bacterial glutathione peroxidase from S. aureus, revealing its structure, electron donor system, and atypical catalytic mechanism.

The paper investigates how protein diffusion and proteome stability change in psychrophilic and hyperthermophilic bacteria, finding that proteome dynamics decouple from viability in cold environments.

This study shows that inorganic pyrophosphate disrupts amorphous bone mineral interfaces in hypophosphatasia, leading to brittle fractures and altered mineral structure.

This study investigates the structural dynamics of the HIV-1 gp41 membrane-interacting domain using molecular dynamics simulations and AI-based analysis, revealing diverse conformations of MPER-TMD and interactions with PIP2.

This paper develops Slab-PTM, a module for coarse-grained models to simulate post-translational modifications in intrinsically disordered proteins, showing improved accuracy in phase behavior predictions.