A. F. Stewart

Human Pancreatic α-Cell Heterogeneity and Trajectory Inference Analysis Using Integrated Single Cell- and Single Nucleus-RNA Sequencing Platforms

This study identifies human α-cell and β-cell subtypes and trajectories using integrated scRNA-seq and snRNA-seq, revealing gene signatures for interconversion and highlighting in vivo transitions in T2D.

Single Cell RNA-Seq Analysis of Regenerative Drug-Treated Human Pancreatic Islets Identifies A Cycling Alpha Cell Population As Key Beta Cell Progenitors

This study uses single-cell RNA sequencing to identify cycling alpha cells as key beta cell progenitors in response to DYRK1A inhibitor treatment in human pancreatic islets.

The BOD1L subunit of the SETD1A complex sustains the expression of DNA damage repair genes despite restraining H3K4 trimethylation

The paper shows that BOD1L in the SETD1A complex maintains DNA repair gene expression despite reducing H3K4 trimethylation, and its loss causes DNA damage and cell death in ESCs.

Single Nucleus RNA Sequencing of Human Pancreatic Islets In Vitro and In Vivo Identifies New Gene Sets and Three β-Cell Subpopulations with Different Transcriptional Profile

This study compares scRNA-seq and snRNA-seq of human pancreatic islets, identifying new gene markers and three β-cell subpopulations with distinct transcriptional profiles.

Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice

This study generates multi-chemokine receptor reporter mice to analyze receptor expression dynamics on myeloid cells, revealing high specificity and selectivity in chemokine receptor expression.

Human Beta Cell Mass Expansion In Vivo With A Harmine and Exendin-4 Combination: Quantification and Visualization By iDISCO+ 3D Imaging

This study shows a combination of DYRK1A inhibitor and GLP1R agonist increases human beta cell mass in vivo by 400-700% in mice, using iDISCO+ 3D imaging for quantification.

The Set1 complex is dimeric and acts with Jhd2 demethylation to convey symmetrical H3K4 trimethylation

This paper shows that the yeast Set1 complex is dimeric, leading to symmetric H3K4me3, and that Jhd2 demethylase removes asymmetric H3K4me3, working together to establish epigenetic symmetry.