Author

D. Ginsburg

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Cell Biology· 3 Evolutionary Biology· 1 Pathology· 1 Physiology· 1

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 6 Approach:experimental assay· 6 Organism:human· 6 Assay:proteomics· 6 Organism:mouse· 3 Approach:genetic perturbation / CRISPR· 2 Research Focus:cell signaling & trafficking· 1 Approach:deep learning· 1

Frequent Co-Authors

B. T. Emmer· 4 shared V. T. Tang· 4 shared A. Yee· 3 shared R. Khoriaty· 3 shared A. Nesvizhskii· 2 shared A.-C. Gingras· 2 shared C. A. Kretz· 2 shared D. R. Siemieniak· 2 shared

Activity by Year

2017-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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4 shared papersCell Biology

B. T. Emmer

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Identification of LMAN1 and SURF4 dependent secretory cargoes

V. T. Tang, P. S. Abbineni, F. da Veiga Leprevost, V. Basrur, B. T. Emmer, A. Nesvizhskii, D. Ginsburg

The paper identifies secretory cargoes dependent on LMAN1 and SURF4 using CRISPR knockout and mass spectrometry in HuH7 cells, revealing distinct cargo repertoires and complex recognition mechanisms.

bioRxiv · Apr 6, 2023Cell Biology

Hepatic inactivation of murine Surf4 results in marked reduction in plasma cholesterol

V. T. Tang, J. McCormick, B. Xu, Y. Wang, H. Fang, X. Wang, D. Siemieniak, R. Khoriaty, B. T. Emmer, X.-W. Chen, D. Ginsburg

This study shows that reducing SURF4 in the liver decreases PCSK9 and increases LDLR, leading to lower plasma cholesterol despite reduced lipoprotein secretion.

bioRxiv · Aug 1, 2022Physiology

Deep mutational scanning and massively parallel kinetics of plasminogen activator inhibitor-1 functional stability

L. M. Haynes, Z. M. Huttinger, A. Yee, C. A. Kretz, D. R. Siemieniak, D. A. Lawrence, D. Ginsburg

The paper analyzes PAI-1 variants to identify mutations that increase functional stability and inhibitory activity, and develops a method to measure functional half-lives of hundreds of variants in parallel.

bioRxiv · Jul 19, 2022 · PreprintBiochemistry

Identification of secreted proteins by comparison of protein abundance in conditioned media and cell lysates.

P. S. Abbineni, V. T. Tang, F. da Veiga Leprevost, V. Basrur, J. Xiang, A. I. Nesvizhskii, D. Ginsburg

The paper identifies secreted proteins by comparing their abundance in conditioned media and cell lysates, finding proteins enriched in media as potential secreted candidates.

bioRxiv · Jun 16, 2022Biochemistry

Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape

Z. M. Huttinger, L. M. Haynes, A. Yee, C. A. Kretz, D. R. Siemieniak, D. Ginsburg

The paper uses deep mutational scanning to assess the functional impact of amino acid substitutions in PAI-1, identifying variants that disrupt its inhibitory activity against uPA.

bioRxiv · Apr 15, 2021Biochemistry

Phage Display Broadly Identifies Inhibitor-reactive Regions in von Willebrand Factor

A. Yee, M. Dai, S. E. Croteau, J. A. Shavit, S. W. Pipe, F. Meng, D. Ginsburg

This study uses phage display and NGS to identify inhibitor-reactive regions in von Willebrand factor, revealing distinct patterns among patients and overlap with a commercial antibody.

bioRxiv · Mar 8, 2021Pathology

Murine Surf4 is essential for early embryonic development

B. T. Emmer, P. J. Lascuna, E. Kotnik, T. Saunders, R. Khoriaty, D. Ginsburg

This study shows that Surf4 is essential for early embryonic development in mice, as Surf4-/- embryos die between E3.5 and E9.5, while Surf4+/- mice are normal.

bioRxiv · Feb 7, 2019Cell Biology

The cargo receptor SURF4 promotes the efficient cellular secretion of PCSK9

B. T. Emmer, G. G. Hesketh, E. Kotnik, V. T. Tang, P. J. Lascuna, J. Xiang, A.-C. Gingras, X.-W. Chen, D. Ginsburg

This study identifies SURF4 as a cargo receptor that facilitates PCSK9 secretion by linking it to COPII components and confirming its functional role through multiple experimental approaches.

bioRxiv · May 31, 2018Cell Biology

The Function of the COPII Gene Paralogs SEC23A and SEC23B Are Interchangeable In Vivo

R. Khoriaty, G. Hesketh, A. Bernard, A. C. Weyand, D. Mellacheruvu, G. Zhu, M. J. Hoenerhoff, B. McGee, L. Everett, E. J. Adams, B. Zhang, T. Saunders, A. Nesvizhskii, D. J. Klionsky, J. A. Shavit, , D. Ginsburg

This study shows SEC23A and SEC23B are functionally interchangeable in vivo, suggesting phenotypic differences arise from gene expression rather than protein function.

bioRxiv · Nov 7, 2017Evolutionary Biology